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Table 3 Hepatic gene expression levelsa

From: Tetradecylthiopropionic acid induces hepatic mitochondrial dysfunction and steatosis, accompanied by increased plasma homocysteine in mice

Gene symbol

Gene name

Control diet

0.9 % TTP diet

P-value

PPAR co-activators:

  Pparg1a

Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha

1.00 ± 0.32

1.56 ± 0.33

0.021

  Ppara

Peroxisome proliferator-activated receptor alpha

1.00 ± 0.37

1.12 ± 0.23

0.468

  Pparg

Peroxisome proliferator-activated receptor gamma, coactivator 1 beta

1.00 ± 0.78

1.57 ± 0.31

0.040

Fatty acid mobilization, uptake, and secretion:

  CD36

Peroxisome proliferator-activated receptor gamma, coactivator 1 beta

1.00 ± 0.64

3.24 ± 0.67

<0.001

  Lipe

Hormone sensitive lipase

1.00 ± 0.16

1.24 ± 0.24

0.036

  Lipc

Hepatic lipase

1.00 ± 0.14

0.93 ± 0.15

0.384

  Fabp1

Fatty acid binding protein 1, liver

1.00 ± 0.35

1.38 ± 0.27

0.039

  Acly

ATP citrate lyase

1.00 ± 0.22

0.97 ± 1.02

0.187

  Apob

Apolipoprotein B

1.00 ± 0.16

0.94 ± 0.14

0.450

  Slc25a20

acylcarnitine translocase

1.00 ± 0.25

1.31 ± 0.44

0.116

Antioxidant activity and inflammation:

  Tnfa

Tumor necrosis factor alpha

1.00 ± 0.44

0.51 ± 0.09

0.014

  Il1b

Interleukin 1, beta

1.00 ± 0.63

0.68 ± 0.32

0.054

  Sod1

Superoxide dismutase 1, soluble

1.00 ± 0.11

1.27 ± 0.22

0.010

  Sod2

Superoxide dismutase 2, mitochondrial

1.00 ± 0.12

1.08 ± 0.11

0.187

Phosphatidyl synthesis and metabolism:

  Chka

Choline kinase alpha

1.00 ± 0.35

0.35 ± 0.14

<0.001

  Chkb

Ethanolamine kinase/Choline kinase beta

1.00 ± 0.18

1.49 ± 0.29

0.001

  Pcyt1a

Phosphate cytidylyltransferase 1, choline, alpha

1.00 ± 0.19

0.80 ± 0.08

0.018

Choline oxidation pathway, mitochondria:

  Chdh

Choline dehydrogenase

1.00 ± 0.35

1.01 ± 0.15

0.941

  Dmgdh

Dimethylglycine dehydrogenase precursor

1.00 ± 0.28

0.65 ± 0.07

0.007

  Sardh

Sarcosine dehydrogenase

1.00 ± 0.31

0.69 ± 0.14

0.032

  Shmt2

Serine hydroxymethyltransferase 2 (mit)

1.00 ± 0.24

0.59 ± 0.10

0.001

Choline oxidation pathway, cytosol:

  Bhmt

betaine-homocysteine S-methyltransferase

1.00 ± 0.76

0.15 ± 0.05

0.012

  Gnmt

Glycine N-methyltransferase

1.00 ± 0.14

0.90 ± 0.15

0.232

Amino acid metabolism and derivatives:

  Mtr/Mr

Methionine synthase (5-methyltetrahydrofolate-homocystein methyltransferase)

1.00 ± 0.22

0.70 ± 0.12

0.006

  Mtrr/Msr

Methionine synthase reductase (5-methyltetrahydrofolate-homocystein)

1.00 ± 0.50

0.68 ± 0.17

0.040

  Msra

Methionine sulfoxide reductase A

1.00 ± 0.50

0.88 ± 0.21

0.556

  Msrb2

Methionine sulfoxide reductase B2

1.00 ± 0.10

0.75 ± 0.14

0.001

  Shmt1

Serine hydroxyl-methyltransferse (soluble)

1.00 ± 0.57

0.66 ± 0.23

0.165

Transsulfuration:

  Cbs

Cystathionine-beta-synthase

1.00 ± 0.46

0.49 ± 0.18

0.017

  Cth

Cystathionase (cystathionine gamma lyase)

1.00 ± 0.51

0.63 ± 0.22

0.121

  1. Abbreviations: TTP tetradecylthiopropionic acid
  2. aExpression levels were normalized to 18 s expression and relative values to control are given as means ± standard deviation (n = 7–8). Results were analyzed by unpaired t-test, and P –values < 0.05 were considered significant
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Lipids in Health and Disease

ISSN: 1476-511X