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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Intracellular high cholesterol content disorders the clock genes, apoptosis-related genes and fibrinolytic-related genes rhythmic expressions in human plaque-derived vascular smooth muscle cells

Fig. 3

Clock genes expressions in the VSMCs after treated with cholesterol or ox-LDL. a. Western-blot analyses of clock genes expressions in VSMCs followed by stimulation with cholesterol for 48 h. b. Relative band intensities of Western-Blot, analyzed using Image J software. Each value was normalized to β-actin levels and was presented as mean ± SD (n = 3). c. Real-time PCR analyses of clock genes expressions in VSMCs followed by stimulation with ox-LDL for 24 h. Each value was normalized to GAPDH mRNA levels and defined the NC levels as 1. Data were presented as mean ± SD (n = 3). d. Western-blot analyses of clock genes expressions in VSMCs followed by stimulation with ox-LDL for 24 h. e. Relative band intensities of Western-Blot, analyzed using Image J software. Each value was normalized to β-actin levels and was presented as mean ± SD (n = 3). Unpaired student’s t test was used to assess the expression differences between groups. *p < 0.05 and **p < 0.01 versus NC

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Lipids in Health and Disease

ISSN: 1476-511X