Table 1 Primer sequences for RT-PCR
From: Sake lees extract improves hepatic lipid accumulation in high fat diet-fed mice
Gene | Primers | Sequence (5′-3′) | Size (bp) | Tm (°C) |
|---|---|---|---|---|
β-actin | Sense | GGACTCCTATGTGGGTGACGAGG | 366 | 55 |
Antisense | GGGAGAGCATAGCCCTCGTAGAT | |||
Peroxisome proliferator-activated receptor γ (PPARγ) | Sense | TCCGTGATGGAAGACCACTCGCAT | 124 | 55 |
Antisense | CAGCAACCATTGGGTCAGCTCTTG | |||
Peroxisome proliferator-activated receptor α (PPARα) | Sense | TCTCCAGCTTCCAGCCCTTCCTCA | 144 | 55 |
Antisense | TTCACATGCGTGAACTCCGTAGTG | |||
Liver X receptor α (LXRα) | Sense | TCCATCAACCACCCCCACGAC | 328 | 63 |
Antisense | CAGCCAGAAAACACCCAACCT | |||
Sterol regulatory element-binding transcription factor 1 (SREBP-1c) | Sense | GTAGGTCACCGTTTCTTTGTGGAC | 163 | 60 |
Antisense | TGGGCTGAGCAATACAGTTCAAC | |||
CD36 | Sense | GAACCACTGCTTTCAAAAACTGG | 102 | 55 |
Antisense | TGCTGTTCTTTGCCACGTCA | |||
Acetyl-CoA carboxylase (ACC) | Sense | AGGAGGGAAAGGGATCAGAA | 435 | 57 |
Antisense | TGTGCTGCAGGAAGATTGAC | |||
Fatty acid synthase (FAS) | Sense | TGGTGGTGTGGACATGGTCACAGA | 160 | 60 |
Antisense | CCGAAGCTGGGGGTCCATTGTGTG | |||
Carnitine palmitoyltransferase I (CPT1) | Sense | CCTGGGCATGATTGCAAAG | 330 | 57 |
Antisense | ACAGACTCCAGGTACCTGCTCA | |||
Phosphoenolpyruvate carboxykinase (PCK1) | Sense | CTCTGATCCAGACCTTCCAA | 105 | 60 |
Antisense | GAAGTCCAGACCGTTATGCAG | |||
Glucose 6-phosphatase (G6Pase) | Sense | GATTGCTGACCTGAGGAACGC | 198 | 59 |
Antisense | ATAGGCACGGAGCTGTTGCTG | |||
Glucokinase (GCK) | Sense | GTGTACAAGCTGCACCCGA | 301 | 58 |
Antisense | CAGCATGCAAGCCTTCTTG | |||
Phosphofructokinase (PFK) | Sense | CGATCTATCTACCTATGCCGACA | 245 | 59 |
Antisense | ACACCCGCATCAATCTCATTCA |