Fig. 4

Increased resistance to chemotherapeutic agents in CerS4-overexpressing MCF-7 cells. (A–C) Cell death following treatment of MCF-7 and CerS4-overexpressing MCF-7 cells (MCF-7/CerS4) with (A) doxorubicin, (B) paclitaxel, or (C) tamoxifen (n = 3). IC50, half-maximal inhibitory concentration. (D) Expression of ATP-binding cassette (ABC) genes in MCF-7/CerS4 cells was assessed by RNA sequencing (n = 3). (E) Relative mRNA levels of ABC transporters (ABCC1, ABCC2, ABCC4, ABCC11, ABCB1, ABCB4, and ABCG2) was assessed by real-time PCR (n = 3). A two-way ANOVA and a Tukey post hoc test was performed for (A, B, and C). The adjusted P-values obtained from DESeq2-analysis for (D). A two-tailed Student’s t-test was performed for (E). Significance is indicated as follows: ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001