Fig. 6
From: Ceramide synthase 4 overexpression exerts oncogenic properties in breast cancer
Downregulation of CerS4 in MCF-7/ADR cells partially reverses multiple drug resistance. (A) mRNA expression of various CerS family members in MCF-7 cells and MCF-7/ADR before and after (MCF-7/ADR + shCerS4) CerS4 downregulation (n = 3). (B) Ceramide levels in MCF-7 and MCF-7/ADR before and after (MCF-7/ADR + shCerS4) CerS4 downregulation using LC-ESI-MS/MS (n = 3). (C–E) Cell death was examined in MCF-7, MCF-7/ADR, and MCF-7/ADR + shCerS4 cells following treatment with (C) doxorubicin, (D) paclitaxel, or (E) tamoxifen (n = 3). IC50, half-maximal inhibitory concentration. A one-way ANOVA was performed for (A and B), and a two-way ANOVA was performed for (C, D, and E), respectively. Additionally, a Tukey post hoc test was conducted for each analysis. Significance is indicated compared to control group; *P < 0.05, **P < 0.01, ***P < 0.001 and compared to MCF-7/ADR group; ##P < 0.01, ###P < 0.001