Fig. 7

CerS4 downregulation in MCF-7/ADR cells partially reverses cancer-related pathway activation and increases ABC gene expression. (A) Representative western blot analysis of the indicated protein levels (left) and their densitometric analysis (right) in MCF-7 cells and MCF-7/ADR before and after (MCF-7/ADR + shCerS4) CerS4 downregulation (n = 3). (B) Relative mRNA levels of ATP-binding cassette (ABC) transporters in MCF-7/ADR cells, using data from GSE24460 (n = 2). (C) Relative mRNA levels of ABC transporters (ABCC1, ABCC2, ABCC4, ABCB1, and ABCG2) in MCF-7/ADR cells before and after (MCF-7/ADR + shCerS4) CerS4 downregulation (n = 3). A one-way ANOVA and a Tukey post hoc test was performed for (A and C). The adjusted P-values obtained from limma-analysis for (B). Significance is indicated as follows: ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. Akt, protein kinase B; GSK3β, glycogen synthase kinase-3 beta; mTOR, mammalian target of rapamycin; S6K, ribosomal S6 kinase