Fig. 8
From: Ceramide synthase 4 overexpression exerts oncogenic properties in breast cancer
Downregulation of CerS4 in MCF-7/ADR cells reverses EMT. (A) The number of shared DEG between CerS4-overexpressing MCF-7 cells (MCF-7/CerS4) and MCF-7/ADR. (B) EMT score in MCF-7/ADR cells, calculated using data from GSE24460 (n = 2). (C, D) Representative western blots of an epithelial marker (E-cadherin) and mesenchymal markers (N-cadherin, vimentin, and Snail) (C) and their densitometric analysis (D) in MCF-7 cells and MCF-7/ADR cells before and after (MCF-7/ADR + shCerS4) CerS4 downregulation (n = 3). (E) Cell migration in MCF-7, MCF-7/ADR, and MCF-7/ADR + shCerS4 cells (n = 3). A two-tailed Student’s t-test was performed for (B). A one-way ANOVA and a Tukey post hoc test was performed for (D). Significance is indicated as follows: *P < 0.05, **P < 0.01, and ***P < 0.001