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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: High-fat stimulation induces atrial neural remodeling by reducing NO production via the CRIF1/eNOS/P21 axi

Fig. 3

PA-stimulated generation of ROS regulates SIRT1/eNOS expression. (A) NO content in the supernatant after PA stimulation. Detection of SIRT1 and eNOS protein levels after PA stimulation using WB. Detection of SIRT1 and eNOS protein levels was performed using WB after scavenging ROS with NAC (5 mM). (D) After overexpressing SIRT1, the levels of SIRT1 and eNOS proteins were detected, along with the measurement of NO levels in the supernatant. After silencing SIRT1, an analysis was conducted on the levels of SIRT1 and eNOS proteins, and the levels of NO in the supernatant. (F) Levels of eNOS acetylation after overexpression of SIRT1 or silencing of SIRT1

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Lipids in Health and Disease

ISSN: 1476-511X