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Fig. 7 | Lipids in Health and Disease

Fig. 7

From: High-fat stimulation induces atrial neural remodeling by reducing NO production via the CRIF1/eNOS/P21 axi

Fig. 7

NO inhibits proliferation by nitrosylating Keap1 to promote Nrf2 entry into the nucleus to induce P21 transcription. (A, B) NO content and protein expression of Nrf2 and P21 in the supernatant after stimulation of PC12 neuronal cells using 0, 10, 50, 100, 200, and 500 µM SNAP. (C) Immunofluorescence detection of Nrf2 protein expression after the addition of 500µM SANP. Scale bar = 20 μm. (D) Protein expression of Nrf2 and P21 after silencing the expression of Nrf2 after adding 500µM SNAP. (E) Detection of nitrosylation of Keap1 after adding 500µM SNAP. (F, G) Upon PA stimulation, after overexpression of eNOS in upper cardiomyocytes and silencing of Nrf2 in lower neuronal cells, WB detected P21, PGP9.5, and TH protein expression, and EdU showed the proportion of neuronal cell proliferation (20X). Scale bar = 100 μm

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Lipids in Health and Disease

ISSN: 1476-511X