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Fig. 11 | Lipids in Health and Disease

Fig. 11

From: Role of arachidonic acid metabolism in intervertebral disc degeneration: identification of potential biomarkers and therapeutic targets via multi-omics analysis and artificial intelligence strategies

Fig. 11

Inhibiting AKR1C3 using AH6809 treats IVDD. A A box plot illustrates the functional similarity of the five hub genes, as revealed through Friends analysis. AKR1C3 exhibits the highest degree of correlation with other genes. B AKR1C3 expression in NPCs transfected with an NC negative control (empty plasmid) or an AKR1C3 overexpression plasmid, as detected by RT-qPCR. Data are expressed as mean ± SD (n = 3) (*, P < 0.05; **, P < 0.01; ***, P < 0.001). C Correlation between AKR1C3 and various biological processes. D, E Apoptosis in NPCs transfected with a control NC plasmid or an AKR1C3 overexpression plasmid, as detected by flow cytometry. Data are expressed as mean ± SD (n = 3) (*, P < 0.05; **, P < 0.01; ***, P < 0.001). F Changes in gene expression of anabolic markers (ACAN and COL2A1) and catabolic markers (MMP-3, ADAMTS5, and COX2), as determined by RT-qPCR. Data are expressed as mean ± SD (n = 3) (*, P < 0.05; **, P < 0.01; ***, P < 0.001)

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Lipids in Health and Disease

ISSN: 1476-511X