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Fig. 10 | Lipids in Health and Disease

Fig. 10

From: A comprehensive multiomics approach reveals that high levels of sphingolipids in cardiac cachexia adipose tissue are associated with inflammatory and fibrotic changes

Fig. 10

Experimental verification. A, B Contents of Cer and S1P in adipose tissue. C, D Contents of IL-6 and TNF-α in adipose tissue. E, F Contents of IL-6 and TNF-α in plasma. G-J Protein expression of S1PR1, S1PR2, and SPHK measured by Western blot in adipose tissue. K-M mRNA expression of monocyte/macrophage chemotaxis genes, including Ccl5, Mdk and Mmp14. N-P mRNA expression of representative fibrosis- associated genes, including Col1, Col6, and Col14. Q, R mRNA expression of Tgf-β/Smad signalling pathway genes, including Tgf-β and Smad2. S, T Representative images of HE staining and fat cell inner diameter of adipose tissue. U, V Representative images of Sirius scarlet staining and positive area of adipose tissue. W, X Representative images of immunostaining and positive areas of CD68 in adipose tissue. Scale bar = 100 μm. Data are mean ± SD, n = 6. **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. the control group

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Lipids in Health and Disease

ISSN: 1476-511X