Table 1 Primers sequence of RT-qPCR
Gene | Sequence(5’-3’) |
|---|---|
β-actin | Forward 5’-AGCCATGTACGTAGCCATCC’ Reverse 5’-CTCTCAGCTGTGGTGGTGAA’ |
Col1 | Forward 5’-TGGTCCTGCTGGCAAGAATGG’ Reverse 5’-TCTGTCACCTTGTTCGCCTGTC’ |
Col6 | Forward 5’-CCCACGCAGAACAACCGAATTG’ Reverse 5’-TCCTTGATGCCCACAGAAACTACC’ |
Col14 | Forward 5’-CCGTGACTTCAGTTCTCCAGACAG’ Reverse 5’-TCCAGGCACCATAACCACTTCTTC’ |
Tgf-β | Forward 5’-CAAGTCAACTGTGGAGCAAC’ Reverse 5’-AACCCAGGTCCTTCCTAAAG’ |
Smad2 | Forward 5’-CAAGGCGATCGAGAACTGCG’ Reverse 5’-GCCGTCTACAGTGAGTGAGG’ |
Ccl5 | Forward 5’-ACACCACTCCCTGCTGCTTTG’ Reverse 5’-TCTCTGGGTTGGCACACACTTG’ |
Mdk | Forward 5’-TTCTAGCCCTTGTTGCCCTCTTG’ Reverse 5’-ACACTCGCTGCCCTTCTTCAC’ |
Mmp14 | Forward 5’-TGAGGAGGAGACGGAGGTGATC’ Reverse 5’-CAGTACCAGGAGCAGCAGCAG’ |