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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Puerarin ameliorates metabolic dysfunction-associated fatty liver disease by inhibiting ferroptosis and inflammation

Fig. 3

Puerarin inhibits hepatic ferroptosis and activates the SIRT1/Nrf2 signaling pathway in MAFLD mice. (A) The protein levels of SIRT1, p-Nrf2, Nrf2, HO-1, SLC7A11, and GPX4 were determined by Western blotting. (B) Representative images of immunohistochemical staining showing the expression of SIRT1 in liver sections. (C) Molecular docking model of SIRT1 and Puerarin (i. Spherical diagram, ii. 3D structures of the binding interface, iii. Puerarin was predicted to interact with SIRT1 via the amino acids PRO271, GLN345, ASN346, HIS363, GLN294, LYS444, and VAL445 via hydrophobic interactions or hydrogen bonds. The data are expressed as the mean ± SD (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001

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Lipids in Health and Disease

ISSN: 1476-511X