Fig. 4

Puerarin increases Nrf2 nuclear translocation and reduces liver inflammation in MAFLD mice. (A) Representative images of immunofluorescence staining of p-Nrf2. Scale Bar, 20 μm. (B) F4/80, IL-1β, and IL-6 were determined by immunohistochemical staining. Scale Bar, 50 μm. (C) The protein levels of p-p65/p65, TNF-α, IL-1β, and IL-6 were determined by Western blotting. (D) Hepatic mRNA levels of Tnf-α, Il-1β, and Il-6 were determined by RT‒qPCR. The data are expressed as the mean ± SD (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001