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Fig. 5 | Lipids in Health and Disease

Fig. 5

From: Puerarin ameliorates metabolic dysfunction-associated fatty liver disease by inhibiting ferroptosis and inflammation

Fig. 5

Puerarin inhibits ferroptosis, activates the SIRT1/Nrf2 signaling pathway, and reduces inflammation in PA-induced AML12 cells in vitro. (A) Coimmunoprecipitation was performed to assess the interaction between SIRT1 and Nrf2 in AML12 cells. (B) Protein levels of SIRT1, p-Nrf2, Nrf2, HO-1, SLC7A11, and GPX4 in AML12 cells in the Control + PBS, Control + Puerarin, PA + PBS, and PA + Puerarin groups. (C) Coimmunoprecipitation was performed to assess the interaction between SIRT1 and p65 in AML12 cells. (D) Protein levels of p-p65/p65, TNF-α, IL-1β, and IL-6 in the four groups. (E) Representative images of immunofluorescence staining of p-Nrf2 in AML12 cells. Scale Bar, 20 μm. (F) Representative images of Ferrorange staining in the four groups. Scale Bar, 20 μm. The data are expressed as the mean ± SD (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001

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Lipids in Health and Disease

ISSN: 1476-511X