Skip to main content
Fig. 6 | Lipids in Health and Disease

Fig. 6

From: Puerarin ameliorates metabolic dysfunction-associated fatty liver disease by inhibiting ferroptosis and inflammation

Fig. 6

SIRT1 inhibition reduced the protective effect of puerarin on PA-induced AML12 cells. (A) Protein levels of SIRT1, p-Nrf2, Nrf2, HO-1, SLC7A11, and GPX4 in AML12 cells in the Control + PBS, Control + Puerarin, PA + PBS, PA + Puerarin, PA + EX-527 (10 μM), and PA + Puerarin + EX-527 (10 μM) groups. (B) Representative images of immunofluorescence staining of p-Nrf2 in AML12 cells. Scale Bar, 20 μm. (C) The protein levels of p-p65/p65, TNF-α, IL-1β, and IL-6 in AML12 cells in the six groups. (D) The mRNA levels of Tnf-α, Il-1β, and Il-6 in AML12 cells in the six groups. The data are expressed as the mean ± SD (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X