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Fig. 3 | Lipids in Health and Disease

Fig. 3

From: Discovery of a novel lipid metabolism-related gene signature to predict outcomes and the tumor immune microenvironment in gastric cancer by integrated analysis of single-cell and bulk RNA sequencing

Fig. 3

Data normalization and quality assurance for scRNA-seq. A A total of 400 cells were tested for further investigation after quality control and normalization removed 2 nonconforming cells. B Mitochondrial gene sequences were shown to correlate with sequencing depth. C Analysis was performed on a total of 16,288 genes, 14,788 of which showed little change between cells and 1,500 of which showed significant variance. D PCA calculated using data from scRNA-seq. E and F The marker genes and their expression patterns. G It was determined that 15 PCs had significant differences when P was less than 0.5

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