Fig. 2

Inducible Hif2α deletion in PDGFRβ + cells does not affect adipose tissue remodeling in diet-induced obesity. A Schematic diagram illustrating HFD feeding experiment. Male control or Epas1-bKO mice were kept on normal chow diet until 8 weeks of age before being switched to HFD feeding for another 8 weeks. Mice were i.p. injected with TAM (100 mg/kg) for 5 five consecutive days before the diet switch. B Relative frequency of PDGFRβ + subpopulations within gWAT and iWAT after 8 weeks of HFD feeding. n = 6 per group. C Representative gWAT H&E staining images of control and Epas1-bKO mice after HFD feeding. Scale bar denotes 200 µM. D Average gWAT adipocyte size of control and Epas1-bKO mice after HFD feeding. n = 6 per group. E Inflammation- and fibrosis-related gene expression within gWAT from control and Epas1-bKO mice after HFD feeding. n = 6 per group. F Representative iWAT H&E staining images of control and Epas1-bKO mice after HFD feeding. Scale bar denotes 200 µM. G Average iWAT adipocyte size of control and Epas1-bKO mice after HFD feeding. n = 6 per group. H Inflammation- and fibrosis-related gene expression within iWAT from control and Epas1-bKO mice after HFD feeding. n = 6 per group. I Western blot analysis of phosphorylated AKT (pAKT), total AKT and β-actin in gWAT whole tissue extracts from control and Epas1-bKO mice after HFD feeding. For quantification, intensity of pAKT band is normalized to that of total AKT band, and intensity of total AKT is normalized to that of β-actin. J Western blot analysis of phosphorylated AKT (pAKT), total AKT and β-actin in iWAT whole tissue extracts from control and Epas1-bKO mice after HFD feeding. For quantification, intensity of pAKT band is normalized to that of total AKT band, and intensity of total AKT is normalized to that of β-actin