Fig. 2

Representative immunofluorescence microscopy image detailing the dystrophin border identification identified using the red dystrophin stain (A). Image B shows the dystrophin stain alone, which was used to identify the cell boarder carried out in MATLAB (C). Image D shows the GLUT4 stain (green) including the spots identified by MATLAB analysis in yellow, and this is replicated in E but with the inclusion of the 5 × 1 μm concentric rings that follow the contours of the PM as defined by dystrophin stain. The magenta staining within image A and F represents fibre type staining of MHC1. Scale bar represents 25 μm