Table 1 Oligos used in this study
Oligo name | Sequence | Reference |
|---|---|---|
gRNA1 top | CACCGATGACTCAGCAGCCACTTCG | This study |
gRNA1 bottom | AAACCGAAGTGGCTGCTGAGTCATC | |
gRNA2 top | CACCGTGACACTCGGGACAACCCCA | [34] |
gRNA2 bottom | AAACTGGGGTTGTCCCGAGTGTCAC | |
gRNA3 top | CACCGAAGCAGCTGCTCGTGTTTCC | [35] |
gRNA3 bottom | AAACGGAAACACGAGCAGCTGCTTC | |
Wdr45 sequencing 1Â F | GATACTCTGAGGTATCCTCCAC | This study |
Wdr45 sequencing 1 R | GGAATAGGGTGTCAGGAGAGG | |
Wdr45 sequencing 2Â F | CGGAAGCAAGTGGTTGAGATCC | This study |
Wdr45 sequencing 2 R | CAGGGAAGAGGAACTGACTGTG | |
Wdr45 sequencing 3Â F | GAGCAGAGCTTACTGCAAAGCC | This study |
Wdr45 sequencing 3 R | GCTGCAAGACAGACCCGTAATG |