Multiple roles of dihomo-γ-linolenic acid against proliferation diseases
© Wang et al; licensee BioMed Central Ltd. 2012
Received: 23 December 2011
Accepted: 14 February 2012
Published: 14 February 2012
Considerable arguments remain regarding the diverse biological activities of polyunsaturated fatty acids (PUFA). One of the most interesting but controversial dietary approaches focused on the diverse function of dihomo-dietary γ-linolenic acid (DGLA) in anti-inflammation and anti-proliferation diseases, especially for cancers. This strategy is based on the ability of DGLA to interfere in cellular lipid metabolism and eicosanoid (cyclooxygenase and lipoxygenase) biosynthesis. Subsequently, DGLA can be further converted by inflammatory cells to 15-(S)-hydroxy-8,11,13-eicosatrienoic acid and prostaglandin E1 (PGE1). This is noteworthy because these compounds possess both anti-inflammatory and anti-proliferative properties. PGE1 could also induce growth inhibition and differentiation of cancer cells. Although the mechanism of DGLA has not yet been elucidated, it is significant to anticipate the antitumor potential benefits from DGLA.
KeywordsDihomo-γ-linolenic acid (DGLA) Prostaglandin E1 (PGE1) Proliferation Differentiation Cancer
Supplementation of cell cultures in vitro or feeding animals with ω-3 or ω-6 polyunsaturated fatty acids (PUFAs) led to an increase of these PUFAs in cell membrane phospholipids and may influence membrane properties [1, 2]. Moreover, PUFAs and their metabolites, eicosanoids, are considered as important mediators and modulators of the intracellular network of signals , they change oxidative metabolism and may have a direct effect on gene expression when activating the specific nuclear receptors and transcription factors [3–5]. ω-3 types PUFAs were reported to improve immunological response, prevent proliferation and initiate apoptosis, kill tumor cells in vitro [6–8], and inhibit tumor growth in experimental animals, while ω-6 PUFAs are supposed not to possess such significant antitumor effects even generate opposite action [9, 10].
One of the most interesting but controversial dietary approaches focused on the diverse function of dihomo-dietary γ-linolenic acid (DGLA) in anti-inflammation and anti-proliferation diseases although it is a distinct ω-6 type PUFA [11–13]. To evaluate DGLA-related nutraceuticals critically, it is essential to elucidate the mechanisms underlying the relationship between DGLA and health maintenance. This review will focus on recent studies that address the physiologic functions and mechanisms of function of DGLA in proliferation and hyperplasia diseases.
Dihomo-γ-linolenic acid (DGLA) is a 20-carbon ω-6 polyunsaturated fatty acid (PUFA) derived in vivo from linolenic acid, an essential fatty acid. DGLA can then be converted to arachidonic acid (AA), another 20-carbon ω-6 PUFA [11, 12]. Both DGLA and AA are substrates of the lipid-peroxidizing enzyme COX. Through a series of free radical reactions, COX metabolizes DGLA and AA to form various bioactive metabolites, namely, the 1 and the 2 series of prostaglandins (PGs1 and PGs2), respectively. Unlike PGs2, which are generally viewed as pro-inflammatory, PGs1 actually possess anti-inflammatory and anticancer activities [14–16]. For example, PGE1, one form of PGs1, could inhibit vascular smooth muscle cell proliferation, reduce vascular cell adhesion, and attenuate the development of atherosclerosis [17–19].
The increase in DGLA relative to AA is able to attenuate the biosynthesis of AA metabolites, i.e., 2-series prostaglandins, 4-series leukotrienes and platelet-activating factor (PAF), and exerts an anti-inflammatory effect in human subjects [20–22] (Figure 1). Another research demonstrated that addition of GLA or DGLA together with a mixed D5/D6 fatty-acid desaturase inhibitor CP-24879 inhibited D5 desaturase activity and the further conversion of DGLA into AA. This led to a very substantial increase in the accumulation of DGLA from 2.3% to almost 12% of total fatty acids without a change in the level of AA .
In addition, because addition of DGLA bypasses a key regulatory rate-limiting enzymatic step (Δ6 desaturase) which controls the formation of long-chain PUFA of the (ω-6) series, it may generate a systemic decline in Δ6 desaturation. Researches has confirmed that a reduced capacity to convert LA to DGLA has been associated with various physiologic and pathophysiologic states, including aging, diabetes, alcoholism, atopic dermatitis, premenstrual syndrome, rheumatoid arthritis, cancer and cardiovascular disease [12, 17, 23]. Therefore, supplementation of DGLA may be of value in alleviating some of the symptoms of these various diseases.
DGLA metabolic oxidation and mechanisms against proliferation diseases
COX-2, a rate-limiting enzyme in the pathway of PG synthesis, is one of the interesting cellular factors and has been suggested to be associated with carcinogenesisin colorectal cancer [32–34]. The contribution of COX-2 to tumorigenesis includes an increased resistance to apoptotic stimuli, enhanced invasiveness, and stimulated angiogenesis [14, 32–35]. In addition, expression of COX-2 has also been found to be high in other types of cancers including prostate, breast, gastric, pancreatic, liver, lung, and skin cancers [36–42]. COX enzymes are integralmembrane proteins located in the endoplasmic reticulumand nuclear membrane. These proteins are known to catalyze the rate-limiting step in the metabolism of arachidonic acid (AA), resulting in the production of prostaglandin G2 (cyclooxygenase reaction) [16, 43]. Prostaglandin G2 is then converted to prostaglandin H2 (peroxidase reaction), a target of several specific prostanoid synthases, resulting in the production of prostaglandins, prostacyclins, and thromboxanes [43, 44]. Two COX isoforms have been identified. COX-1 is constitutively expressed in most normal mammalian tissues,where it is involved in the maintenance of tissue homeostasis.In contrast, COX-2 is rapidly expressed as a consequence of stimulation by growth factors, cytokines, inflammatory mediators, and tumor promoters [44, 45]. While a paradoxical aspect of COX-2 is that it could also convert DGLA to PGE1 which possesses anti-inflammation, anti-proliferation and promoting differentiation capacity . It seemed that the ratio of PGE1/PGE2 mediated by COX-2 produced a balance efficancy on cell growth.
The majority of prostaglandins, including PGE1, PGE2, PGI2, PGD2, PGF2, and TXA2, are quickly secreted from cells and bind locally to membrane-bound prostanoid receptors termed EP, IP, DP, FP, and TP, respectively. The EP receptor subclass is further divided into four subtypes EP1, EP2, EP3, and EP4 . With regard to tumor biology, the prostanoid PGE2 stimulates the growth and invasion of several different tumor cells and promotes angiogenesis by increasing VEGF production [47–49]. In comparison, PGE1 possesses distinct anti-inflammation and anti-proliferation action [15, 50]. GLA or DGLA supplementation studies conducted in humans and rodents have shown that the synthesis of 1-series prostaglandins, and not the 2-series prostaglandins (PGE2, derived from AA), is selectively elevated [16, 17]. Although the increases in the tissue levels of PGE1 after DGLA supplementation are modest relative to PGE2, effects are noteworthy because select biological properties of PGE1 are ~20 times stronger than PGE2. In particular, PGE1 elicits an array of intracellular responses by binding to select G protein coupled surface PGE (EP) receptors and/or the prostacyclin (IP) receptor . Four subtypes of the EP-receptor, termed EP1, EP2, EP3 and EP4 have been identified. The EP2, EP4 and IP receptors couple to adenylatecyclase via a Gs-protein, and receptor activation results in increases in intracellular levels of cyclic 3',5'-adenosine monophosphate (cAMP) [52, 53]. This in turn stimulates the expression of numerous genes through the PKA-mediated phosphorylation of the nuclear CREB binding proteins. The transcriptional co-activator, CBP, in turn, mediates PKA-induced transcription by binding to the PKA phosphorylated activation domain of CREB. CREB proteins can also heterodimerize with other members of the b-ZIP or basic zipper family of transcription factors, including Fos proteins (c-fos, Fosb, Fra-1, Fra-2), and Jun proteins (c-jun, JunB, JunD). Elevation of cAMP stimulates the expression of numerous genes through the protein kinase A (PKA)-mediated phosphorylation of the nuclear cAMP response element binding proteins (CREB). Through this mechanism, PGE1 has been shown to inhibit vascular smooth muscle cell (SMC) proliferation in vitro [53, 54]. It is significant because reducing the migration and proliferation of vascular SMC could arrest the formation of the typical atherosclerotic plaque [55, 56]. The question of whether PGE1 and PGE2 act on the same or different receptors is not yet resolved. Some reports provide data to demonstrate that PGE1 binds to the PGE2 receptors EP2 and EP4 [57, 58], indicating that PGE1 could compete with PGE2 through binding to the same receptors.
More encouraging researches indicate that several diseases may benefit from GLA or DGLA administration [12, 17, 20–22]. It is reported that GLA or DGLA suppresses human synovial cell proliferation in culture by increasing PGE1 synthesis and intracellular cAMP levels . In addition, administration of GLA or DGLA is capable of suppressing human T-cell proliferation [12, 60]. Although the precise mechanism of responses remains to be investigated, it has been suggested that the incorporation of select diet-derived PUFA may alter the dynamic lipid environment that influences protein lateral diffusion of anchored receptors, thereby modulating their function [2, 3]. These findings highlight the need for establishing a therapeutic GLA or DGLA dose that will down-regulate autoimmune and cell-mediated responses.
Efficacy of DGLA against tumors
A growing number of studies suggest that DGLA is unique among the (n-6) PUFA family members (LA, GLA and AA) in its potential to suppress tumor growth and metastasis. DGLA has the ability to inhibit both motility and invasiveness of human colon cancer cells by increasing the expression of E-cadherin, a cell-to-cell adhesion molecule that acts as a suppressor of metastasis [65, 66]. In addition, DGLA reduces tumor-endothelium adhesion, a key factor in the establishment of distant metastases, partly by improving gap junction communication within the endothelium [65, 67]. These encouraging results indicate that further investigations should be given priority.
Since PUFAs enhance free radical generation and lipid peroxidation process and thus, induce apoptosis, it suggests that these events lead to depletion of ATP levels in the tumor cells which brings about their death. This implies that an interaction exists among fatty acids, lipid peroxidation process, apoptosis and genes/oncogenes that regulate apoptotic process [6–10]. Researches previously have showed that some polyunsaturated fatty acids (PUFAs) induced apoptosis of tumor cells with little or no cytotoxic action on normal cells under certain conditions [6, 68, 69]. It was observed that of all the fatty acids tested, GLA or DGLA was the most effective in selectively killing the tumor cells . In a co-culture experiment wherein normal human skin fibroblasts and human breast cancer cells were grown together in a petri dish supplemented with GLA or DGLA, only human breast cancer cells were eliminated without any effect on normal skin fibroblasts [65, 68, 70, 71]. These results reconfirmed that GLA or DGLA show selective tumoricidal action at least in vitro. These molecular changes were found to be significantly associated with enhanced degree of lipid peroxidation in the fatty acid supplemented tumor cells [6, 65, 68–71]. GLA and DGLA were also found to be capable of suppressing the expression of oncogenes Her-2/neu and Bcl-2 and enhance p53 activity and thus, induce apoptosis of tumor cells [72–74]. In an extension of these studies, it was noted that cyclooxygenase and lipoxygenase inhibitors blocked the tumoricidal action of DGLA on human cervical carcinoma; whereas anti-oxidants inhibited cytotoxic action of DGLA on human breast cancer cells [6, 73, 75–77]. Prostaglandins (PGE1) from DGLA mediated by COX inhibited the growth of HeLa cells [77, 78]. LOX products were more potent than PGs in inhibiting of HeLa cell growth which was confirmed by the observation that a 9-fold increased formation of hydroxides occurred in HeLa cells . These results suggest that both COX products, LOX products and free radicals, lipid peroxidation are involved in the tumoricidal action of DGLA. A significant increase in the formation of free radicals and lipid peroxides was noted only in tumor cells treated with DGLA compared to untreated tumor cells [6–10, 75–78], suggesting that the involvement of COX and LOX products, free radicals and lipid peroxides in the tumoricidal action of DGLA varies depending on the cell type that is being tested. Recent studies demonstrated that the longer-chain n-6 PUFA produced by GLA feeding decreased the Th2 cytokine and immunoglobulin (Ig)G1 antibody response. GLA and DGLA could also induce T-regulatory cell activity, e.g., transforming growth factor (TGF)-beta-producing T cells, and reduce proinflammatory interleukin (IL)-1 and tumor necrosis factor (TNF)-alpha production , indicating immunity mechanism is likely to participate in the anti-tumor effect.
Drug resistance is a major issue in the management of cancer. Hence, methods or strategies to prevent and reverse tumor cell drug resistance are needed. More studies confirmed that GLA and DGLA could kill drug resistant tumor cells in vitro and augment tumoricidal actions of anti-cancer drugs synergistically [6, 65, 68–71]. But, it is not clear as to the exact mechanism by which this synergism between anti-cancer drugs and DGLA occurs. The possible mechanism lies in DGLA could augment uptake and decrease efflux of anti-cancer drugs and thus, reverse tumor cell drug resistance [6, 75].
However, the mechanism of action mediating DGLA effects was not fully elucidated, because feeding of GLA or DGLA as ethyl esters or triglycerides (triacylglycerols) generally led to only a small increase in GLA or DGLA content in cell membrane lipids, often accompanied by a very significant increase in AA content. Furthermore, the ratio of PGE1/PGE2 produced in cells obtained from animals fed with GLA-containing oils was shown to be substantially lower than the cellular ratio of DGLA/AA [16, 17]. Similarly, in studies with cultured mouse fibrosarcoma cells rendered deficient in essential fatty acids and then replenished with either DGLA or AA, the ratio of synthesized PGE1/PGE2 was considerably smaller than the cellular ratio of DGLA/AA [80, 81]. It should, however, be pointed out that in both in vivo dietary study and in vitro cell culture study, the cellular content of AA, even after significant enrichment with DGLA, was still 2.5 ± 3-fold higher than DGLA, mainly due to effective desaturation of DGLA to AA [16, 81, 82]. Therefore, an effective solution to accumulate the content of DGLA is to administer selective Δ5 desaturase inhibitors to stop DGLA from further converting to AA. Sesame, curcumin and other analog desaturase inhibitors, for example, CP-24879, a d5/d6 desaturase inhibitor, have been verified to exert anti-proliferation or anti-cancer effect by enhancing the concentration of DGLA [16, 82–84].
While studies showed in some cancer cells, after using the COX and LOX inhibitor, GLA or DGLA only led to a partially growth inhibition of cancer cells, which indicates that the change ratio of PGE1/PGE2 derived from DGLA metabolites couldn't completely account for the antitumor effect [6, 12, 16, 17]. Recent research has verified that free radicals and lipid perioxidation play an important role in the cytotoxic action to cancer, which could be completely arrested by anti-oxidants such as vitamin E and SOD depending on different cell lines . On the other hand, some reports assume that vitamin E only partially arrests the growth inhibition of cancer cells mediated by PUFAs [70, 73, 85]. One of the reasons for the different experimental results may originate from various employed cell lines. Another reason is that near all the cytotoxicity effects against cancer cells depend on the time and concentrition of the metabolic products from PUFA. Therefore, DGLA is likely to exert its antitumor cytotoxic effects from free radicals and lipid perioxidation and metabolites, which probably inhibit the proliferation, promote apoptosis and even make cancer cell differentiate and maturation [6–10] (Figure 3). Further investigation need to confirm the presumption.
Possible adverse effects of oral supplementation with DGLA
A previous report noted that administration of DGLA ethyl ester to health volunteer (1 g/d) reduced platelet aggregation . However, DGLA ethyl ester is an experimental reagent and is not available for practical and nutritional usage. On January 27, 1993, the United States Court of Appeals for the Seventh Circuit ruled that GLA, containing oil is a single food ingredient and therefore not subject to food additive regulation. As a result of this legislation, GLA-containing oils (primrose oil, blackcurrant seed oil and borage oil) have become increasingly popular with retailers and are being sold as encapsulated supplements . To evaluate DGLA-related nutraceuticals critically, it is essential to elucidate the mechanisms underlying the relationship between DGLA and health maintenance. Recent researches indicated that from rodent to human, oral supplementation of DGLA-enriched oil showed no-observed-adverse-effect. In acute and subchronic oral toxicity tests in rats, DGLA oil, ranging from 500, 1000, 2000 mg/kg to 10 g/kg was orally administered. There was no death in either sex and no toxicological changes in body weight, food consumption, ophthalmological examination, urinalysis, hematological examination, blood biochemical examination, necropsy, organ weight, or histopathological examination . The researcher also compared the dietary effects of DGLA with GLA on the fatty acid composition. The resulted showed that the DGLA concentrations in the liver, serum, and brain of the DGLA group were higher than those of the GLA oil group. The DGLA levels in the liver, serum, and brain significantly increased with increasing dosage of DGLA in the diet. DGLA administration significantly increased the ratio of PGE1/PGE2 in the rat plasma. These results suggest that the dietary effect of DGLA would be more dominant than GLA . Further research confirmed the safety of consumption of DGLA in human. DGLA-enriched oil (50 or 150 mg as free DGLA) was administered to healthy men for 4 weeks. The DGLA content in serum phospholipids dose-dependently increased and returned to the initial level after a 4-week washout. No side effects or changes in platelet aggregation were observed. These results indicate that oral supplementation with DGLA oil can safely increase serum DGLA content . Undoubtedly, elucidation of the mechanism(s) of DGLA will lead to the establishment of dietary guidelines designed to reduce the incidence and severity of inflammatory or hyperproliferative diseases.
In summary, regulation of DGLA administration may have multiple biological benefits for a series of diseases associated with proliferation. DGLA exert anti-proliferation action through enhancing free radicals, lipid perioxidation, PGE1 and 15-HETrE synthesis, which directly or indirectly influence the cell growth, apoptosis and differentiation mediated by downstream signal transduction cascades. The consumption of GLA or administration of DGLA may offer new strategies for treatment and prevention of proliferation and hyperplasia diseases.
cAMP Response element binding proteins
Platelet activating factor
Reactive oxygen species
Protein kinase A
Polyunsaturated fatty acids
Smooth muscle cell.
This work is supported by Scientific Research Program Funded by Shaanxi Provincial Education Department (No.2007JK233, 2010JK484), the Key Project of Ministry of Education of China (No.205002) and the National Natural Science Foundation of China (No.81172135).
- Zhang B, Wang P, Zhou Q, Chen C, Zhuo S, Ye Y, He Q, Chen Y, Su Y: The relationships between erythrocyte membrane n-6 to n-3 polyunsaturated fatty acids ratio and blood lipids and C-reactive protein in Chinese adults: an observational study. Biomed Environ Sci. 2011, 24: 234-242.PubMedGoogle Scholar
- Gomolka B, Siegert E, Blossey K, Schunck WH, Rothe M, Weylandt KH: Analysis of omega-3 and omega-6 fatty acid-derived lipid metabolite formation in human and mouse blood samples. Prostaglandins Other Lipid Mediat. 2011, 94: 81-87. 10.1016/j.prostaglandins.2010.12.006View ArticlePubMedGoogle Scholar
- Das UN: Essential Fatty acids - a review. Curr Pharm Biotechnol. 2006, 7: 467-482. 10.2174/138920106779116856View ArticlePubMedGoogle Scholar
- Das UN: Essential fatty acids: biochemistry, physiology and pathology. Biotechnol J. 2006, 1: 420-439. 10.1002/biot.200600012View ArticlePubMedGoogle Scholar
- Zietemann V, Kröger J, Enzenbach C, Jansen E, Fritsche A, Weikert C, Boeing H, Schulze MB: Genetic variation of the FADS1 FADS2 gene cluster and n-6 PUFA composition in erythrocyte membranes in the European Prospective Investigation into Cancer and Nutrition-Potsdam study. Br J Nutr. 2010, 104: 1748-1759. 10.1017/S0007114510002916View ArticlePubMedGoogle Scholar
- Das UN, Madhavi N: Effect of polyunsaturated fatty acids on drug-sensitive and resistant tumor cells in vitro. Lipids Health Dis. 2011, 10: 159- 10.1186/1476-511X-10-159PubMed CentralView ArticlePubMedGoogle Scholar
- Berquin IM, Edwards IJ, Kridel SJ, Chen YQ: Polyunsaturated fatty acid metabolism in prostate cancer. Cancer Metastasis Rev. 2011, 30: 295-309. 10.1007/s10555-011-9299-7View ArticlePubMedGoogle Scholar
- Das UN: Can essential fatty acids reduce the burden of disease(s)?. Lipids Health Dis. 2008, 7: 9- 10.1186/1476-511X-7-9PubMed CentralView ArticlePubMedGoogle Scholar
- Funahashi H, Satake M, Hasan S, Sawai H, Newman RA, Reber HA, Hines OJ, Eibl G: Opposing effects of n-6 and n-3 polyunsaturated fatty acids on pancreatic cancer growth. Pancreas. 2008, 36: 353-362. 10.1097/MPA.0b013e31815ccc44View ArticlePubMedGoogle Scholar
- Astorg P: Dietary N-6 and N-3 polyunsaturated fatty acids and prostate cancer risk: a review of epidemiological and experimental evidence. Cancer Causes Control. 2004, 15: 367-386.View ArticlePubMedGoogle Scholar
- Ruan KH, Cervantes V, So SP: Engineering of a novel hybrid enzyme: an anti-inflammatory drug target with triple catalytic activities directly converting arachidonic acid into the inflammatory prostaglandin E2. Protein Eng Des Sel. 2009, 22: 733-740. 10.1093/protein/gzp058PubMed CentralView ArticlePubMedGoogle Scholar
- Kapoor R, Huang YS: Gamma linolenic acid: an antiinflammatory omega-6 fatty acid. Curr Pharm Biotechnol. 2006, 7: 531-534. 10.2174/138920106779116874View ArticlePubMedGoogle Scholar
- Nasrollahzadeh J, Siassi F, Doosti M, Eshraghian MR, Shokri F, Modarressi MH, Mohammadi-Asl J, Abdi K, Nikmanesh A, Karimian SM: The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression. Lipids Health Dis. 2008, 7: 45- 10.1186/1476-511X-7-45PubMed CentralView ArticlePubMedGoogle Scholar
- Dommels YE, Haring MM, Keestra NG, Alink GM, van Bladeren PJ, van Ommen B: The role of cyclooxygenase in n-6 and n-3 polyunsaturated fatty acid mediated effects on cell proliferation, PGE(2) synthesis and cytotoxicity in human colorectal carcinoma cell lines. Carcinogenesis. 2003, 24: 385-392. 10.1093/carcin/24.3.385View ArticlePubMedGoogle Scholar
- Tabolacci C, Lentini A, Provenzano B, Gismondi A, Rossi S, Beninati S: Similar antineoplastic effects of nimesulide, a selective COX-2 inhibitor, and prostaglandin E1 on B16-F10 murine melanoma cells. Melanoma Res. 2010, 20: 273-279. 10.1097/CMR.0b013e328339d8acView ArticlePubMedGoogle Scholar
- Levin G, Duffin KL, Obukowicz MG, Hummert SL, Fujiwara H, Needleman P, Raz A: Differential metabolism of dihomo-gamma-linolenic acid and arachidonic acid by cyclo-oxygenase-1 and cyclo-oxygenase-2: implications for cellular synthesis of prostaglandin E1 and prostaglandin E2. Biochem J. 2002, 365: 489-496. 10.1042/BJ20011798PubMed CentralView ArticlePubMedGoogle Scholar
- Fan YY, Chapkin RS: Importance of dietary gamma-linolenic acid in human health and nutrition. J Nutr. 1998, 128: 1411-1414.PubMedGoogle Scholar
- Li JH, Yang P, Li AL, Wang Y, Ke YN, Li XL: Cardioprotective effect of liposomal prostaglandin E1 on a porcine model of myocardial infarction reperfusion no-reflow. J Zhejiang Univ Sci B. 2011, 12: 638-643.PubMed CentralView ArticlePubMedGoogle Scholar
- Gupta V, Davis M, Hope-Weeks LJ, Ahsan F: PLGA microparticles encapsulating prostaglandin E1-hydroxypropyl-β-cyclodextrin (PGE1-HPβCD) complex for the treatment of pulmonary arterial hypertension (PAH). Pharm Res. 2011, 28: 1733-1749. 10.1007/s11095-011-0409-6View ArticlePubMedGoogle Scholar
- de Goede J, Verschuren WM, Boer JM, Kromhout D, Geleijnse JM: Alpha-linolenic acid intake and 10-year incidence of coronary heart disease and stroke in 20, 000 middle-aged men and women in the Netherlands. PLoS One. 2011, 6: e17967- 10.1371/journal.pone.0017967PubMed CentralView ArticlePubMedGoogle Scholar
- Bolton-Smith C, Woodward M, Tavendale R: Evidence for age-related differences in the fatty acid composition of human adipose tissue, independent of diet. Eur J Clin Nutr. 1997, 51: 619-624. 10.1038/sj.ejcn.1600455View ArticlePubMedGoogle Scholar
- Johnson MM, Swan DD, Surette ME, Stegner J, Chilton T, Fontech AN, Chilton FH: Dietary supplementation with γ-linolenic acid alters fatty acid content and eicosanoid production in healthy humans. J Nutr. 1997, 127: 1435-1444.PubMedGoogle Scholar
- Shen X, Dannenberger D, Nuernberg K, Nuernberg G, Zhao R: Trans-18:1 and CLA isomers in rumen and duodenal digesta of bulls fed n-3 and n-6 PUFA-based diets. Lipids. 2011, 46: 831-841. 10.1007/s11745-011-3586-5View ArticlePubMedGoogle Scholar
- McNamara RK, Jandacek R, Rider T, Tso P, Cole-Strauss A, Lipton JW: Differential effects of antipsychotic medications on polyunsaturated fatty acid biosynthesis in rats: Relationship with liver delta6-desaturase expression. Schizophr Res. 2011, 129: 57-65. 10.1016/j.schres.2011.03.006PubMed CentralView ArticlePubMedGoogle Scholar
- Senadheera SD, Turchini GM, Thanuthong T, Francis DS: Effects of dietary α-linolenic acid (18:3n-3)/linoleic acid (18:2n-6) ratio on fatty acid metabolism in Murray cod (Maccullochella peelii peelii). J Agric Food Chem. 2011, 59: 1020-1030. 10.1021/jf104242yView ArticlePubMedGoogle Scholar
- Kim HW, Rao JS, Rapoport SI, Igarashi M: Regulation of rat brain polyunsaturated fatty acid (PUFA) metabolism during graded dietary n-3 PUFA deprivation. Prostaglandins Leukot Essent Fatty Acids. 2011, 85: 361-368. 10.1016/j.plefa.2011.08.002PubMed CentralView ArticlePubMedGoogle Scholar
- Borgeat P, Hamberg M, Samuelsson S: Transformation of arachidonic acid and homo-gamma-linolenic acid by rabbit polymorphonuclear leukocytes. Monohydroxy acids from novel lipoxygenase. J Biol Chem. 1976, 251: 7816-7820.PubMedGoogle Scholar
- Simopoulos AP: The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med (Maywood). 2008, 233: 674-688. 10.3181/0711-MR-311. 10.3181/0711-MR-311View ArticleGoogle Scholar
- Skuladottir GV, Heidarsdottir R, Arnar DO, Torfason B, Edvardsson V, Gottskalksson G, Palsson R, Indridason OS: Plasma n-3 and n-6 fatty acids and the incidence of atrial fibrillation following coronary artery bypass graft surgery. Eur J Clin Invest. 2011, 41: 995-1003. 10.1111/j.1365-2362.2011.02497.xView ArticlePubMedGoogle Scholar
- Williams CD, Whitley BM, Hoyo C, Grant DJ, Iraggi JD, Newman KA, Gerber L, Taylor LA, McKeever MG, Freedland SJ: A high ratio of dietary n-6/n-3 polyunsaturated fatty acids is associated with increased risk of prostate cancer. Nutr Res. 2011, 31: 1-8. 10.1016/j.nutres.2011.01.002View ArticlePubMedGoogle Scholar
- Hofmanová J, Vaculová A, Kozubík A: Polyunsaturated fatty acids sensitize human colon adenocarcinoma HT-29 cells to death receptor-mediated apoptosis. Cancer Lett. 2005, 218: 33-41. 10.1016/j.canlet.2004.07.038View ArticlePubMedGoogle Scholar
- Dupertuis YM, Meguid MM, Pichard C: Colon cancer therapy: new perspectives of nutritional manipulations using polyunsaturated fatty acids. Curr Opin Clin Nutr Metab Care. 2007, 10: 427-432. 10.1097/MCO.0b013e3281e2c9d4View ArticlePubMedGoogle Scholar
- Chamras H, Ardashian A, Heber D, Glaspy JA: Fatty acid modulation of MCF-7 human breast cancer cell proliferation, apoptosis and differentiation. J Nutr Biochem. 2002, 13: 711-716. 10.1016/S0955-2863(02)00230-9View ArticlePubMedGoogle Scholar
- Astorg P: Dietary N-6 and N-3 polyunsaturated fatty acids and prostate cancer risk: a review of epidemiological and experimental evidence. Cancer Causes Control. 2004, 15: 367-386.View ArticlePubMedGoogle Scholar
- Cathcart MC, O'Byrne KJ, Reynolds JV, O'Sullivan J, Pidgeon GP: COX-derived prostanoid pathways in gastrointestinal cancer development and progression: Novel targets for prevention and intervention. Biochim Biophys Acta. 2012, 1825: 49-63.PubMedGoogle Scholar
- Zhong B, Cai X, Yi X, Zhou A, Chen S, Su B: In vitro and in vivo effects of a cyclooxygenase-2 inhibitor nimesulide analog JCC76 in aromatase inhibitors-insensitive breast cancer cells. J Steroid Biochem Mol Biol. 2011, 126. 10.18.View ArticlePubMedGoogle Scholar
- Thiel A, Mrena J, Ristimäki A: Cyclooxygenase-2 and gastric cancer. Cancer Metastasis Rev. 2011, 30: 387-395. 10.1007/s10555-011-9312-1View ArticlePubMedGoogle Scholar
- Talar-Wojnarowska R, Gasiorowska A, Olakowski M, Lampe P, Smolarz B, Romanowicz-Makowska H, Malecka-Panas E: Role of cyclooxygenase-2 gene polymorphisms in pancreatic carcinogenesis. World J Gastroenterol. 2011, 17: 4113-4117. 10.3748/wjg.v17.i36.4113PubMed CentralView ArticlePubMedGoogle Scholar
- Sung YK, Hwang SY, Kim JO, Bae HI, Kim JC, Kim MK: The correlation between cyclooxygenase-2 expression and hepatocellular carcinogenesis. Mol Cells. 2004, 17: 35-38.PubMedGoogle Scholar
- Mendes RA, Carvalho JF, Waal I: An overview on the expression of cyclooxygenase-2 in tumors of the head and neck. Oral Oncol. 2009, 45: e124-e128. 10.1016/j.oraloncology.2009.03.016View ArticlePubMedGoogle Scholar
- Sun L, Zhang S, Jiang Z, Huang X, Wang T, Huang X, Li H, Zhang L: Triptolide inhibits COX-2 expression by regulating mRNA stability in TNF-α-treated A549 cells. Biochem Biophys Res Commun. 2011, 416: 99-105. 10.1016/j.bbrc.2011.11.004View ArticlePubMedGoogle Scholar
- Smith WL, DeWitt DL, Garavito RM: Cyclooxygenases: structural, cellular, and molecular biology. Annu Rev Biochem. 2000, 69: 145-182. 10.1146/annurev.biochem.69.1.145View ArticlePubMedGoogle Scholar
- Garavito RM, DeWitt DL: The cyclooxygenase isoforms: structural insights into the conversion of arachidonic acid to prostaglandins. Biochim Biophys Acta. 1999, 1441: 278-287.View ArticlePubMedGoogle Scholar
- Das UN: COX-2 inhibitors and metabolism of essential fatty acids. Med Sci Monit. 2005, 11: RA233-237.PubMedGoogle Scholar
- Rundhaug JE, Simper MS, Surh I, Fischer SM: The role of the EP receptors for prostaglandin E(2) in skin and skin cancer. Cancer Metastasis Rev. 2011, 30: 465-480. 10.1007/s10555-011-9317-9PubMed CentralView ArticlePubMedGoogle Scholar
- Luo H, Chen Z, Jin H, Zhuang M, Wang T, Su C, Lei Y, Zou J, Zhong B: Cyclooxygenase-2 up-regulates vascular endothelial growth factor via a protein kinase C pathway in non-small cell lung cancer. J Exp Clin Cancer Res. 2011, 30: 6- 10.1186/1756-9966-30-6PubMed CentralView ArticlePubMedGoogle Scholar
- Fujino H, Toyomura K, Chen XB, Regan JW, Murayama T: Prostaglandin E2 regulates cellular migration via induction of vascular endothelial growth factor receptor-1 in HCA-7 human colon cancer cells. Biochem Pharmacol. 2011, 81: 379-387. 10.1016/j.bcp.2010.11.001View ArticlePubMedGoogle Scholar
- Liu H, Yang Y, Xiao J, Lv Y, Liu Y, Yang H, Zhao L: COX-2-mediated regulation of VEGF-C in association with lymphangiogenesis and lymph node metastasis in lung cancer. Anat Rec (Hoboken). 2010, 293: 1838-1846. 10.1002/ar.21240.View ArticleGoogle Scholar
- Gao Y, Xu P, Chen L, Li Y: Prostaglandin E1 encapsulated into lipid nanoparticles improves its anti-inflammatory effect with low side-effect. Int J Pharm. 2010, 387: 263-271. 10.1016/j.ijpharm.2009.12.019View ArticlePubMedGoogle Scholar
- Fan YY, Ramos KS, Chapkin RS: Dietary lipid source alters murine macrophage/vascular smooth muscle cell interactions in vitro. J Nutr. 1996, 126: 2083-2088.PubMedGoogle Scholar
- Negishi M, Sugimoto Y, Ichikawa A: Prostanoid receptors and their biological actions. Prog Lipid Rez. 1993, 32: 417-434. 10.1016/0163-7827(93)90017-Q.View ArticleGoogle Scholar
- Fan YY, Ramos KS, Chapkin RS: Cell cycle-dependent inhibition of DNA synthesis in vascular smooth muscle cells by prostaglandin E1: relationship to intracellular cAMP levels. Prostaglandins Leukot Essent Fatty Acids. 1996, 54: 101-107. 10.1016/S0952-3278(96)90066-6View ArticlePubMedGoogle Scholar
- Foulkes NS, Sasone-Corsi P: (199) Transcription factors coupled to the cAMPsignalling pathway. Biochim Biophys Acta. 1996, 1288: 101-121.Google Scholar
- Takai S, Jin D, Kawashima H, Kimura M, Shiraishi-Tateishi A, Tanaka T, Kakutani S, Tanaka K, Kiso Y, Miyazaki M: Anti-atherosclerotic effects of dihomo-gamma-linolenic acid in ApoE-deficient mice. J Atheroscler Thromb. 2009, 16: 480-489. 10.5551/jat.No430View ArticlePubMedGoogle Scholar
- Das UN: Essential fatty acids and their metabolites could function as endogenous HMG-CoA reductase and ACE enzyme inhibitors, anti-arrhythmic, anti-hypertensive, anti-atherosclerotic, anti-inflammatory, cytoprotective, and cardioprotective molecules. Lipids Health Dis. 2008, 7: 37- 10.1186/1476-511X-7-37PubMed CentralView ArticlePubMedGoogle Scholar
- Iyú D, Jüttner M, Glenn JR, White AE, Johnson AJ, Fox SC, Heptinstall S: PGE1 and PGE2 modify platelet function through different prostanoid receptors. Prostaglandins Other Lipid Mediat. 2011, 94: 9-16. 10.1016/j.prostaglandins.2010.11.001View ArticlePubMedGoogle Scholar
- Kojima F, Yuhki K, Kashiwagi H, Ushikubi F: Prostaglandin E1, E2 and their metabolites. Nihon Rinsho. 2010, 68: 20-24.PubMedGoogle Scholar
- Sawamura H, Hayashi H, Onozaki K: Differential effects of prostaglandin E1 and prostaglandin E2 on growth and differentiation of murine myeloid leukemic cell line, M1. Microbiol Immunol. 1995, 39: 809-815.View ArticlePubMedGoogle Scholar
- Ziboh VA, Naguwa S, Vang K, Wineinger J, Morrissey BM, Watnik M: Gershwin ME:Suppression of leukotriene B4 generation by ex-vivo neutrophils isolated from asthma patients on dietary supplementation with gammalinolenic acid-containing borage oil: possible implication in asthma. Clin Dev Immunol. 2004, 11: 13-21. 10.1080/10446670410001670445PubMed CentralView ArticlePubMedGoogle Scholar
- Celotti F, Durand T: The metabolic effects of inhibitors of 5-lipoxygenase and of cyclooxygenase 1 and 2 are an advancement in the efficacy and safety of anti-inflammatory therapy. Prostaglandins Other Lipid Mediat. 2003, 71: 147-162. 10.1016/S1098-8823(03)00039-XView ArticlePubMedGoogle Scholar
- Gregor JI, Kilian M, Heukamp I, Kiewert C, Kristiansen G, Schimke I, Walz MK, Jacobi CA, Wenger FA: Effects of selective COX-2 and 5-LOX inhibition on prostaglandin and leukotriene synthesis in ductal pancreatic cancer in Syrian hamster. Prostaglandins Leukot Essent Fatty Acids. 2005, 73: 89-97. 10.1016/j.plefa.2005.04.016View ArticlePubMedGoogle Scholar
- Cho Y, Ziboh VA: A novel 15-hydroxyeicosatrienoic acid-substituted diacylglycerol (15-HETrE-DAG) selectively inhibits epidermal protein kinase C-beta. Biochim Biophys Acta. 1997, 1349: 67-71.View ArticlePubMedGoogle Scholar
- Ziboh VA, Miller CC, Cho Y: Significance of lipoxygenase-derived monohydroxy fatty acids in cutaneous biology. Prostaglandins Other Lipid Mediat. 2000, 63: 3-13. 10.1016/S0090-6980(00)00093-9View ArticlePubMedGoogle Scholar
- Jiang WG, Hiscox S, Bryce RP, Horrobin DF, Mansel RE: The effects of n-6 polyunsaturated fatty acids on the expression of nm-23 in human cancer cells. Br J Cancer. 1998, 77: 731-738. 10.1038/bjc.1998.120PubMed CentralView ArticlePubMedGoogle Scholar
- Watkins G, Martin TA, Bryce R, Mansel RE, Jiang WG: Gamma-Linolenic acid regulates the expression and secretion of SPARC in human cancer cells. Prostaglandins Leukot Essent Fatty Acids. 2005, 72: 273-278. 10.1016/j.plefa.2004.12.004View ArticlePubMedGoogle Scholar
- Jiang WG, Singhrao SK, Hiscox S, Hallett MB, Bryce RP, Horrobin DF, Puntis MC, Mansel RE: Regulation of desmosomal cell adhesion in human tumour cells by polyunsaturated fatty acids. Clin Exp Metastasis. 1997, 15: 593-602. 10.1023/A:1018435229087View ArticlePubMedGoogle Scholar
- Menéndez JA, Ropero S, del Barbacid MM, Montero S, Solanas M, Escrich E, Cortés-Funes H, Colomer R: Synergistic interaction between vinorelbine and gamma-linolenic acid in breast cancer cells. Breast Cancer Res Treat. 2002, 72: 203-219. 10.1023/A:1014968415759View ArticlePubMedGoogle Scholar
- Ge H, Kong X, Shi L, Hou L, Liu Z, Li P: Gamma-linolenic acid induces apoptosis and lipid peroxidation in human chronic myelogenous leukemia K562 cells. Cell Biol Int. 2009, 33: 402-410. 10.1016/j.cellbi.2009.01.014View ArticlePubMedGoogle Scholar
- Menendez JA, Ropero S, Mehmi I, Atlas E, Colomer R, Lupu R: Overexpression and hyperactivity of breast cancer-associated fatty acid synthase (oncogenic antigen-519) is insensitive to normal arachidonic fatty acid-induced suppression in lipogenic tissues but it is selectively inhibited by tumoricidal alpha-linolenic and gamma-linolenic fatty acids: a novel mechanism by which dietary fat can alter mammary tumorigenesis. Int J Oncol. 2004, 24: 1369-1383.PubMedGoogle Scholar
- de Antueno R, Elliot M, Ells G, Quiroga P, Jenkins K, Horrobin D: In vivo and in vitro biotransformation of the lithium salt of gamma-linolenic acid by three human carcinomas. Br J Cancer. 1997, 75: 1812-1818. 10.1038/bjc.1997.309PubMed CentralView ArticlePubMedGoogle Scholar
- Menendez JA, Ropero S, Lupu R, Colomer R: Omega-6 polyunsaturated fatty acid gamma-linolenic acid (18:3n-6) enhances docetaxel (Taxotere) cytotoxicity in human breast carcinoma cells: Relationship to lipid peroxidation and HER-2/neu expression. Oncol Rep. 2004, 11: 1241-1252.PubMedGoogle Scholar
- Das UN: Gamma-linolenic acid therapy of human glioma-a review of in vitro, in vivo, and clinical studies. Med Sci Monit. 2007, 13: RA119-131.PubMedGoogle Scholar
- Vousden KH: Apoptosis. p53 and PUMA: a deadly duo. Science. 2005, 309: 1685-1686. 10.1126/science.1118232View ArticlePubMedGoogle Scholar
- Das UN, Madhavi N, Sravan Kumar G, Padma M, Sangeetha P: Can tumour cell drug resistance be reversed by essential fatty acids and their metabolites?. Prostaglandins Leukot Essent Fatty Acids. 1998, 58: 39-54. 10.1016/S0952-3278(98)90128-4View ArticlePubMedGoogle Scholar
- de Kock M, Lottering ML, Grobler CJ, Viljoen TC, le Roux M, Seegers JC: The induction of apoptosis in human cervical carcinoma (HeLa) cells by gamma-linolenic acid. Prostaglandins Leukot Essent Fatty Acids. 1996, 55: 403-411. 10.1016/S0952-3278(96)90123-4View ArticlePubMedGoogle Scholar
- Sagar PS, Das UN, Koratkar R, Ramesh G, Padma M, Kumar GS: Cytotoxic action of cis-unsaturated fatty acids on human cervical carcinoma (HeLa) cells: relationship to free radicals and lipid peroxidation and its modulation by calmodulin antagonists. Cancer Lett. 1992, 63: 189-198. 10.1016/0304-3835(92)90260-3View ArticlePubMedGoogle Scholar
- Sagar PS, Das UN: Cytotoxic action of cis-unsaturated fatty acids on human cervical carcinoma (HeLa) cells in vitro. Prostaglandins Leukot Essent Fatty Acids. 1995, 53: 287-299. 10.1016/0952-3278(95)90129-9View ArticlePubMedGoogle Scholar
- Harbige LS: Fatty acids, the immune response, and autoimmunity: a question of n-6 essentiality and the balance between n-6 and n-3. Lipids. 2003, 38: 323-341. 10.1007/s11745-003-1067-zView ArticlePubMedGoogle Scholar
- Rubin D, Laposata M: Cellular interactions between n-6 and n-3 fatty acids: a mass analysis of fatty acid elongation/desaturation, distribution among complex lipids, and conversion to eicosanoids. J Lipid Res. 1992, 33: 1431-1440.PubMedGoogle Scholar
- Rubin D, Laposata M: Regulation of agonist-induced prostaglandin E1 versus prostaglandin E2 production. A mass analysis J Biol Chem. 1991, 266: 23618-23623.PubMedGoogle Scholar
- Fujimoto A, Shingai Y, Oyama TB, Kawanai T, Hashimoto E, Koizumi K, Kimura K, Masuda T, Oyama Y: Apoptosis-inducing action of two products from oxidation of sesamol, an antioxidative constituent of sesame oil: a possible cytotoxicity of oxidized antioxidant. Toxicol In Vitro. 2010, 24: 1720-1726. 10.1016/j.tiv.2010.05.013View ArticlePubMedGoogle Scholar
- Lee CC, Liu KJ, Wu YC, Lin SJ, Chang CC, Huang TS: Sesamin inhibits macrophage-induced vascular endothelial growth factor and matrix metalloproteinase-9 expression and proangiogenic activity in breast cancer cells. Inflammation. 2011, 34: 209-221. 10.1007/s10753-010-9226-zView ArticlePubMedGoogle Scholar
- Risé P, Ghezzi S, Levati MG, Mirtini R, Colombo C, Galli C: Pharmacological modulation of fatty acid desaturation and of cholesterol biosynthesis in THP-1 cells. Lipids. 2003, 38: 841-846. 10.1007/s11745-003-1134-5View ArticlePubMedGoogle Scholar
- Kikawa KD, Herrick JS, Tateo RE, Mouradian M, Tay JS, Pardini RS: Induced oxidative stress and cell death in the A549 lung adenocarcinoma cell line by ionizing radiation is enhanced by supplementation with docosahexaenoic acid. Nutr Cancer. 2010, 62: 1017-1024. 10.1080/01635581.2010.492084View ArticlePubMedGoogle Scholar
- Kernoff PB, Willis AL, Stone KJ, Davies JA, McNicol GP: Antithrombotic potential of dihomo-gamma-linolenic acid in man. Br Med J. 1977, 2: 1441-1444. 10.1136/bmj.2.6100.1441PubMed CentralView ArticlePubMedGoogle Scholar
- Kawashima H, Toyoda-Ono Y, Suwa Y, Kiso Y: Subchronic (13-week) oral toxicity study of dihomo-gamma-linolenic acid (DGLA) oil in rats. Food Chem Toxicol. 2009, 47: 1280-1286. 10.1016/j.fct.2009.03.001View ArticlePubMedGoogle Scholar
- Umeda-Sawada R, Fujiwara Y, Ushiyama I, Sagawa S, Morimitsu Y, Kawashima H, Ono Y, Kiso Y, Matsumoto A, Seyama Y: Distribution and metabolism of dihomo-gamma-linolenic acid (DGLA, 20:3n-6) by oral supplementation in rats. Biosci Biotechnol Biochem. 2006, 70: 2121-2130. 10.1271/bbb.60057View ArticlePubMedGoogle Scholar
- Teraoka N, Kawashima H, Shiraishi-Tateishi A, Tanaka T, Nakamura J, Kakutani S, Kiso Y: Oral supplementation with dihomo-gamma-linolenic acid-enriched oil altered serum fatty acids in healthy men. Biosci Biotechnol Biochem. 2009, 73: 1453-1455. 10.1271/bbb.90112View ArticlePubMedGoogle Scholar
- Zurier RB, Rossetti RG, Jacobson EW, DeMarco DM, Liu NY, Temming JE, White BM, Laposata M: Gamma-linolenic acid treatment of rheumatoid arthritis. A randomized, placebo-controlled trial. Arthritis Rheum. 1996, 39: 1808-1817. 10.1002/art.1780391106View ArticlePubMedGoogle Scholar
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