Correlation between n-3 polyunsaturated fatty acids consumption and BDNF peripheral levels in adolescents
© Ferreira et al.; licensee BioMed Central Ltd. 2014
Received: 13 December 2013
Accepted: 20 February 2014
Published: 5 March 2014
Although several studies have reported an association between mental disorders and serum levels of brain-derived neurotrophic factor (BDNF), this association is still poorly understood. The study of factors associated with both BDNF levels and mental disorders, such as n-3 polyunsaturated fatty acids (n-3 PUFAs), may help to elucidate the mechanisms mediating the relationship between the two variables. Therefore, the present study aimed to evaluate whether the intake n-3 PUFAs correlates with serum levels of BDNF.
This study involved 137 adolescents drawn from a community sample, including a group with high levels of anxiety, assessed using the Screen for Children and Anxiety Related Emotional Disorders. Blood samples were collected and serum BDNF levels were measured. n-3 PUFAs were estimated using a food frequency questionnaire for adolescents. Correlations were performed to assess the association between n-3 PUFAs intake and BDNF levels. Effects of potential confounders (total fat consumption, age, gender and anxiety) were examined using linear regression models. There was a direct correlation between n-3 PUFAs consumption and serum BDNF levels, which remained significant even after accounting for potential confounders.
We were able to detect a correlation between n-3 PUFAs intake and peripheral BDNF levels. Our study was limited by its small sample size, and our external validity may be restricted by the oversampling of anxious adolescents. Our findings may help determine the nature of the association between mental disorders and serum levels of BDNF. However, more studies are needed to elucidate the possible mechanisms by which n-3 PUFAs intake affects BDNF levels, and how this may lead to an increased vulnerability to psychiatric disorders.
KeywordsAnxiety Children Adolescents SCARED BDNF
Brain-Derived Neurotrophic Factor (BDNF) is a dimeric protein thought to be involved in neuronal survival and synaptic plasticity, and to be an important biomarker for psychiatric conditions such as depression and bipolar disorder[1–3]. It is a member of the growth factor family and acts as a regulator of synaptic plasticity, synaptogenesis, as well as neuronal survival and differentiation[4–6]. Therefore, the understanding of processes associated with both BDNF levels and mental disorders may ultimately help determine the underlying mechanisms responsible for the association between these two variables.
Studies of human subjects have demonstrated that lower per capita fish/seafood consumption, which can be used as a surrogate measurement of n-3 polyunsaturated fatty acids (PUFAs) dietary intake, is associated with a higher prevalence of bipolar spectrum disorders[7, 8], major depression[9, 10] and postpartum depression[11, 12]. Experimental studies in rats demonstrate that polyunsaturated fatty acids (PUFAs) could modify brain BDNF levels[13–15] and may play an important role in the function and structure of many membrane proteins[16, 17]. Nevertheless, there is still a need to investigate whether the relationship between dietary n-3 PUFAs intake and BDNF translates to humans. In light of these above findings, the aim of the present study was to evaluate whether the consumption of n-3 PUFAs correlates with serum BDNF levels in humans.
The current study involved 137 adolescents drawn from a community sample, including a group with high rates of anxiety (64.6% female; mean age 13.9 years, SD = 2.42), as assessed by the Screen for Children and Anxiety Related Emotional Disorders (SCARED). A detailed description of the sampling process can be found elsewhere. Briefly, all quartiles of the distribution of SCARED scores were equally represented in the sample (i.e., no to very low anxiety, mild anxiety, moderate anxiety and severe anxiety), although there was an oversampling of participants in the upper quartile (severe anxiety). The SCARED inventory comprises 38 items that can be grouped into subscales according to the different anxiety symptoms investigated, and consists of a screening tool for DMS-IV childhood anxiety disorder. The present study was approved by the Research Ethics Committee of the Hospital de Clínicas de Porto Alegre (GPPG/HCPA, protocol number 08–481). Informed consent was provided by all primary caretakers, and all adolescents assented to participate in the study.
Blood samples were collected between 7 and 10 am after a fasting period of 10–12 hours, centrifuged for 5 minutes at 4500 rpm, and serum was stored at −80°C in order to measure BDNF levels. All BDNF measurements were performed on the same day by sandwich-ELISA using monoclonal antibodies specific for BDNF (R&D Systems, Minneapolis, Minnesota), according to the manufacturer’s instructions. The intra-assay and inter-assay coefficients of variation were 3.7 and 8.5%, respectively. n-3 PUFAs intake was estimated by a food frequency questionnaire administered to the adolescents (FFQ). The quantitative analysis of macro and micronutrient intake was performed using the NutriBase® software (Version NB7 Network) [Phoenix, AZ, USD]. Spearman correlations were performed to assess the association between n-3 PUFA intake and BDNF levels. The effects of potential confounders (total fat intake, age, gender and symptoms of anxiety) were examined using linear regression models (involving log-transformed BDNF values, logBDNF).
We were able to show that n-3 PUFAs intake is associated with serum BDNF levels in adolescents, corroborating to the suggestion that the relationship between mental disorders and BDNF may be mediated by the intake of nutrients such as n-3 PUFAs. This may influence the cellular and physiological processes involved in psychiatric/mood disorders, such as the fatty acid composition of cellular membranes, the modulation of behavioral systems, neurodevelopment, and oxidative stress in specific brain areas[21–25].
Studies suggest that n-3 PUFA-depleted rodents exhibit changes in emotional status such as elevated aggression, depression and anxiety[26–31]. Moreover, the dietary deprivation of n-3 PUFAs in rats may lead to changes in the expression of BDNF in the frontal cortex, in cAMP response element binding protein (CREB) activity and reduce p38 mitogen-activated protein kinase (MAPK) activity.
It is noteworthy that the food frequency questionnaire used in the study contained a varied list of 94 foods or drinks[20, 32]. Thus, the corresponding food sources to the n-3 PUFAs consumption among students were diverse, including vegetable oils and products/preparations based in oils (e.g. biscuits, mayonnaise and fried foods), according to calculations performed with the values depicted in the American Food Table. Besides fish and oils consumption, red meat is also considered a source of n-3 PUFAs, and our region is especially known to have a high consumption of meat. A national survey conducted in both urban and rural areas throughout the country with analysis of individual food consumption in Brazil from 2008–2009, reported that the Southern region of Brazil, including the State of Rio Grande do Sul, where the city Porto Alegre lays, was identified as one of the leading regions in the consumption of red or pork meat. Besides, it was also observed that among 10 to 13 years old population, the n-3 PUFAs displayed a high daily consumption (mean: 1.5 g) in this region, compared to other Brazilian regions. Meat intake is traditionally high and typical from our region, which could probably corroborate to the high n-3 PUFAs consumption levels evidenced in this study.
It is important to note that our study is limited by its small sample size, and that the oversampling of anxious adolescents may restrict our external validity. However, in spite of these limitations, we were able to detect a correlation between n-3 PUFAs consumption and serum BDNF levels. The extent to which peripheral BDNF levels are representative of central nervous system BDNF levels remains to be investigated, although positive correlations between serum and cortical BDNF levels have been reported in preclinical studies, and cellular and behavioral brain functions have been found to be modulated by peripheral BDNF. Furthermore, serum BDNF levels have been associated with neuronal integrity in healthy subjects and decreased BDNF peripheral levels (i.e. serum or plasma) have been consistently found in patients with mood disorders[39, 40]. BDNF is implicated in a variety of neural processes and is related to neuronal developmental stages in both animals and humans. Lang, Hellweg, Sseifert, Schubert and Gallinat, 2007 reported that serum BDNF protein concentrarion might reflect some aspects of neuronal plasticity in the living human brain, playing a role as regulators of neuronal survival and differentiation. Similar findings have been reported by Poo, 2001. Recent studies demonstrate that decreased peripheral BDNF levels are consistently related to disease activity and progression in bipolar disorder as well as to the cognitive impairment seen in patients following their first psychotic episode. More studies are needed to elucidate the possible mechanisms by which n-3 PUFAs consumption affects BDNF levels and influences the vulnerability to psychiatric and mood disorders, especially during certain life stages such as adolescence.
Brain-derived neurotrophic factor
- DMS IV:
Diagnostic and statistical manual of mental disorders IV
Enzyme-linked immunosorbent assay
- n-3 PUFAs:
Omega-3 polyunsaturated fatty acids
Polyunsaturated fatty acids
Revolutions per minute
- SCARED scale:
Screen for children and anxiety related emotional disorders scale.
We thank the children and families for their participation. Without them, this research would not have been possible. We also thank the research teams of the Centro Colaborador em Alimentação e Nutrição Escolar (CECANE – UFRGS) and the Fundo Nacional para o Desenvolvimento da Educação do Ministério da Educação (FNDE).
Role of funding source
CNPq, FIPE-HCPA and FAPERGS provided the funding for the clinicians and interviewers involved in the study, and for the purchase of all necessary materials. CAPES (CFF and GAS), FAPERGS (GAS) and CNPq (FK, PPS, CD, GGM, MZG) provided scholarships to the researchers. No funding agencies participated in the execution of the present study, including data collection, analysis and manuscript preparation.
- Shimizu E, Hashimoto K, Okamura N, Koike K, Komatsu N, Kumakiri C, Nakazato M, Watanabe H, Shinoda N, Okada S, Iyo M: Alterations of serum levels of brain-derived neurotrophic factor (BDNF) in depressed patients with or without antidepressants. Biol Psychiatry. 2003, 54: 70-75. 10.1016/S0006-3223(03)00181-1View ArticlePubMedGoogle Scholar
- Kapczinski F, Frey BN, Kauer-Sant'Anna M, Grassi-Oliveira R: Brain-derived neurotrophic factor and neuroplasticity in bipolar disorder. Expert Rev Neurother. 2008, 8: 1101-1113. 10.1586/14737184.108.40.2061View ArticlePubMedGoogle Scholar
- Kauer-Sant'Anna M, Kapczinski F, Andreazza AC, Bond DJ, Lam RW, Young LT, Yatham LN: Brain-derived neurotrophic factor and inflammatory markers in patients with early- vs. late-stage bipolar disorder. Int J Neuropsychopharmacol. 2009, 12: 447-458. 10.1017/S1461145708009310View ArticlePubMedGoogle Scholar
- Park H, Poo MM: Neurotrophin regulation of neural circuit development and function. Nat Rev Neurosci. 2013, 14: 7-23. 10.1038/nrc3653View ArticlePubMedGoogle Scholar
- Manji HK, Drevets WC, Charney DS: The cellular neurobiology of depression. Nat Med. 2001, 7: 541-547. 10.1038/87865View ArticlePubMedGoogle Scholar
- Poo MM: Neurotrophins as synaptic modulators. Nat Rev Neurosci. 2001, 2: 24-32. 10.1038/35049004View ArticlePubMedGoogle Scholar
- Noaghiul S, Hibbeln JR: Cross-national comparisons of seafood consumption and rates of bipolar disorders. Am J Psychiatry. 2003, 160: 2222-2227. 10.1176/appi.ajp.160.12.2222View ArticlePubMedGoogle Scholar
- McNamara RK: Evaluation of docosahexaenoic acid deficiency as a preventable risk factor for recurrent affective disorders: current status, future directions, and dietary recommendations. Prostaglandins Leukot Essent Fatty Acids. 2009, 81: 223-231. 10.1016/j.plefa.2009.05.017View ArticlePubMedGoogle Scholar
- Hibbeln JR: Fish consumption and major depression. Lancet. 1998, 351: 1213-View ArticlePubMedGoogle Scholar
- De Vriese SR, Christophe AB, Maes M: Lowered serum n-3 polyunsaturated fatty acid (PUFA) levels predict the occurrence of postpartum depression: further evidence that lowered n-PUFAs are related to major depression. Life Sci. 2003, 73: 3181-3187. 10.1016/j.lfs.2003.02.001View ArticlePubMedGoogle Scholar
- Shapiro GD, Fraser WD, Séguin JR: Emerging risk factors for postpartum depression: serotonin transporter genotype and omega-3 fatty acid status. Can J Psychiatry. 2012, 57: 704-712.PubMedGoogle Scholar
- da Rocha CM, Kac G: High dietary ratio of omega-6 to omega-3 polyunsaturated acids during pregnancy and prevalence of post-partum depression. Matern Child Nutr. 2012, 8: 36-48. 10.1111/j.1740-8709.2010.00256.xView ArticlePubMedGoogle Scholar
- Rao JS, Ertley RN, Lee HJ, DeMar JC, Arnold JT, Rapoport SI, Bazinet RP: n-3 polyunsaturated fatty acid deprivation in rats decreases frontal cortex BDNF via a p38 MAPK-dependent mechanism. Mol Psychiatry. 2007, 12: 36-46. 10.1038/sj.mp.4001888View ArticlePubMedGoogle Scholar
- Vetrivel U, Ravichandran SB, Kuppan K, Mohanlal J, Das UN, Narayanasamy A: Agonistic effect of polyunsaturated fatty acids (PUFAs) and its metabolites on brain-derived neurotrophic factor (BDNF) through molecular docking simulation. Lipids Health Dis. 2012, 11: 109- 10.1186/1476-511X-11-109PubMed CentralView ArticlePubMedGoogle Scholar
- Ferreira CF, Bernardi JR, Krolow R, Arcego DM, Fries GR, de Aguiar BW, Senter G, Kapczinski FP, Silveira PP, Dalmaz C: Vulnerability to dietary n-3 polyunsaturated fatty acid deficiency after exposure to early stress in rats. Pharmacol Biochem Behav. 2013, 107: 11-19.View ArticlePubMedGoogle Scholar
- Youdim KA, Martin A, Joseph JA: Essential fatty acids and the brain: possible health implications. Int J Dev Neurosci. 2000, 18: 383-399. 10.1016/S0736-5748(00)00013-7View ArticlePubMedGoogle Scholar
- Spector AA, Yorek MA: Membrane lipid composition and cellular function. J Lipid Res. 1985, 26: 1015-1035.PubMedGoogle Scholar
- Isolan L, Salum GA, Osowski AT, Amaro E, Manfro GG: Psychometric properties of the Screen for Child Anxiety Related Emotional Disorders (SCARED) in Brazilian children and adolescents. J Anxiety Disord. 2011, 25: 741-748. 10.1016/j.janxdis.2011.03.015View ArticlePubMedGoogle Scholar
- Salum GA, Isolan LR, Bosa VL, Tocchetto AG, Teche SP, Schuch I, Costa JR, Costa Mde A, Jarros RB, Mansur MA, Knijnik D, Silva EA, Kieling C, Oliveira MH, Medeiros E, Bortoluzzi A, Toazza R, Blaya C, Leistner-Segal S, Salles JF, Silveira PP, Goldani MZ, Heldt E, Manfro GG: The multidimensional evaluation and treatment of anxiety in children and adolescents: rationale, design, methods and preliminary findings. Rev Bras Psiquiatr. 2011, 33: 181-195. 10.1590/S1516-44462011000200015View ArticlePubMedGoogle Scholar
- Willett WC: Nutritional Epidemiology. 1998, Oxford: Oxford University Press,View ArticleGoogle Scholar
- Balanzá-Martínez V, Fries GR, Colpo GD, Silveira PP, Portella AK, Tabarés-Seisdedos R, Kapczinski F: Therapeutic use of omega-3 fatty acids in bipolar disorder. Expert Rev Neurother. 2011, 11: 1029-1047. 10.1586/ern.11.42View ArticlePubMedGoogle Scholar
- Liperoti R, Landi F, Fusco O, Bernabei R, Onder G: Omega-3 polyunsaturated fatty acids and depression: a review of the evidence. Curr Pharm Des. 2009, 15: 4165-4172. 10.2174/138161209789909683View ArticlePubMedGoogle Scholar
- Rombaldi Bernardi J, de Souza ER, Ferreira CF, Pelufo Silveira P: Fetal and neonatal levels of omega-3: effects on neurodevelopment, nutrition, and growth. ScientificWorldJournal. 2012, 2012: 202473-PubMedGoogle Scholar
- Tur JA, Bibiloni MM, Sureda A, Pons A: Dietary sources of omega 3 fatty acids: public health risks and benefits. Br J Nutr. 2012, 107 (Suppl 2): S23-S52.View ArticlePubMedGoogle Scholar
- Williams LL, Kiecolt-Glaser JK, Horrocks LA, Hillhouse JT, Glaser R: Quantitative association between altered plasma esterified omega-6 fatty acid proportions and psychological stress. Prostaglandins Leukot Essent Fatty Acids. 1992, 47: 165-170. 10.1016/0952-3278(92)90155-CView ArticlePubMedGoogle Scholar
- Zimmer L, Hembert S, Durand G, Breton P, Guilloteau D, Besnard JC, Chalon S: Chronic n-3 polyunsaturated fatty acid diet-deficiency acts on dopamine metabolism in the rat frontal cortex: a microdialysis study. Neurosci Lett. 1998, 240: 177-181. 10.1016/S0304-3940(97)00938-5View ArticlePubMedGoogle Scholar
- Fedorova I, Salem N: Omega-3 fatty acids and rodent behavior. Prostaglandins Leukot Essent Fatty Acids. 2006, 75: 271-289. 10.1016/j.plefa.2006.07.006View ArticlePubMedGoogle Scholar
- Zimmer L, Delion-Vancassel S, Durand G, Guilloteau D, Bodard S, Besnard JC, Chalon S: Modification of dopamine neurotransmission in the nucleus accumbens of rats deficient in n-3 polyunsaturated fatty acids. J Lipid Res. 2000, 41: 32-40.PubMedGoogle Scholar
- Zimmer L, Vancassel S, Cantagrel S, Breton P, Delamanche S, Guilloteau D, Durand G, Chalon S: The dopamine mesocorticolimbic pathway is affected by deficiency in n-3 polyunsaturated fatty acids. Am J Clin Nutr. 2002, 75: 662-667.PubMedGoogle Scholar
- Mathieu G, Oualian C, Denis I, Lavialle M, Gisquet-Verrier P, Vancassel S: Dietary n-3 polyunsaturated fatty acid deprivation together with early maternal separation increases anxiety and vulnerability to stress in adult rats. Prostaglandins Leukot Essent Fatty Acids. 2011, 85: 129-136. 10.1016/j.plefa.2011.07.001View ArticlePubMedGoogle Scholar
- Mathieu G, Denis S, Lavialle M, Vancassel S: Synergistic effects of stress and omega-3 fatty acid deprivation on emotional response and brain lipid composition in adult rats. Prostaglandins Leukot Essent Fatty Acids. 2008, 78: 391-401. 10.1016/j.plefa.2008.05.003View ArticlePubMedGoogle Scholar
- Slater B, Philippi ST, Fisberg RM, Latorre MR: Validation of a semi-quantitative adolescent food frequency questionnaire applied at a public school in São Paulo, Brazil. Eur J Clin Nutr. 2003, 57: 629-635. 10.1038/sj.ejcn.1601588View ArticlePubMedGoogle Scholar
- , : National Nutrient Database for Standard Reference, Release 25. 2012,Google Scholar
- Howe PR, Meyer BJ, Record S, Baghurst K: Contribution of red meat to very long chain omega-3 fatty acid (VLCOmega3) intake. Asia Pac J Clin Nutr. 2003, 12: S27-Google Scholar
- , : Pesquisa de Orçamentos Familiares 2008–2009, Análise de Consumo Alimentar no Brasil. 2011, 2011: Instituto Brasileiro de Geografia e Estatística (IBGE),Google Scholar
- Karege F, Schwald M, Cisse M: Postnatal developmental profile of brain-derived neurotrophic factor in rat brain and platelets. Neurosci Lett. 2002, 328: 261-264. 10.1016/S0304-3940(02)00529-3View ArticlePubMedGoogle Scholar
- Schmidt HD, Duman RS: Peripheral BDNF produces antidepressant-like effects in cellular and behavioral models. Neuropsychopharmacology. 2010, 35: 2378-2391. 10.1038/npp.2010.114PubMed CentralView ArticlePubMedGoogle Scholar
- Lang UE, Hellweg R, Seifert F, Schubert F, Gallinat J: Correlation between serum brain-derived neurotrophic factor level and an in vivo marker of cortical integrity. Biol Psychiatry. 2007, 62: 530-535. 10.1016/j.biopsych.2007.01.002View ArticlePubMedGoogle Scholar
- Frey BN, Andreazza AC, Houenou J, Jamain S, Goldstein BI, Frye MA, Leboyer M, Berk M, Malhi GS, Lopez-Jaramillo C, Taylor VH, Dodd S, Frangou S, Hall GB, Fernandes BS, Kauer-Sant'Anna M, Yatham LN, Kapczinski F, Young LT: Biomarkers in bipolar disorder: a positional paper from the International Society for Bipolar Disorders Biomarkers Task Force. Aust N Z J Psychiatry. 2013, 47 (4): 321-332. 10.1177/0004867413478217View ArticlePubMedGoogle Scholar
- Grande I, Kapczinski F, Stertz L, Colpo GD, Kunz M, Cereser KM, Kauer-Sant'Anna M, Frey B, Vieta E, Magalhaes PV: Peripheral brain-derived neurotrophic factor changes along treatment with extended release quetiapine during acute mood episodes: an open-label trial in drug-free patients with bipolar disorder. J Psychiatr Res. 2012, 46: 1511-1514. 10.1016/j.jpsychires.2012.08.017View ArticlePubMedGoogle Scholar
- Fernandes BS, Gama CS, Ceresér KM, Yatham LN, Fries GR, Colpo G, de Lucena D, Kunz M, Gomes FA, Kapczinski F: Brain-derived neurotrophic factor as a state-marker of mood episodes in bipolar disorders: a systematic review and meta-regression analysis. J Psychiatr Res. 2011, 45: 995-1004. 10.1016/j.jpsychires.2011.03.002View ArticlePubMedGoogle Scholar
- Ruiz de Azua S, Matute C, Stertz L, Mosquera F, Palomino A, de la Rosa I, Barbeito S, Vega P, Kapczinski F, González-Pinto A: Plasma brain-derived neurotrophic factor levels, learning capacity and cognition in patients with first episode psychosis. BMC Psychiatry. 2013, 13: 27- 10.1186/1471-244X-13-27View ArticlePubMedGoogle Scholar
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