Potential role of the interaction between equine estrogens, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) in the prevention of coronary heart and neurodegenerative diseases in postmenopausal women
© Perrella et al; licensee BioMed Central Ltd. 2003
Received: 21 May 2003
Accepted: 20 June 2003
Published: 20 June 2003
An inverse relationship between the level of high-density lipoprotein (HDL) and coronary heart disease (CHD) has been reported. In contrast, oxidized HDL (oHDL) has been shown to induce neuronal death and may play an important role in the pathogenesis of CHD. In the present study we have investigated a: the effect of various equine estrogens on HDL oxidation, b: the inhibition of LDL oxidation by HDL and c: the effect of these estrogens on LDL oxidation in the presence of HDL.
All 11 equine estrogens tested protected the HDL from oxidation in a concentration dependant manner. Equilenin, 17β-dihydroequilenin, and 17α-dihydroequilenin (Δ6–8-estrogens) were found to be the most potent inhibitors of HDL oxidation. Some of the novel ring B unsaturated estrogens were 2.5 to 4 times more potent inhibitors of HDL oxidation than 17β-estradiol. HDL was found to delay LDL oxidation. The protection of LDL oxidation by HDL is enhanced by the addition of estrogen, with equilenin being again more potent than 17β-estradiol.
Equine estrogens can differentially inhibit the oxidation of HDL with the Δ6–8-estrogens being the most potent antioxidants. The ability of estrogens to enhance HDL's antioxidant activity is to our knowledge the first report of an interaction of estrogen with HDL that results in the delay or inhibition of LDL oxidation. This may be another mechanism by which estrogens may reduce the risk of CHD and neurodegenerative diseases in healthy and younger postmenopausal women.
Keywordsneurodegenerative diseases coronary heart disease oxidized HDL oxidized LDL postmenopausal women antioxidants conjugated dienes lag time Alzheimer's disease
Oxidatively modified lipoproteins, such as oxidized low-density lipoprotein (oLDL) have been implicated in the pathogenesis of atherosclerosis and neurodegenerative diseases such as Alzheimer's disease (AD) [1–5]. This peroxidation of the low-density lipoprotein (LDL) molecule renders it immunogenic and causes monocyte recruitment, foam cell formation and cytotoxicity to various cells including neurons [6–8].
In contrast, epidemiological data has suggested a strong inverse correlation between plasma HDL concentrations and the incidence of coronary and cerebral atherosclerosis [9, 10]. Traditionally, this relationship has been proposed to be due in part to the involvement of HDL in reverse cholesterol transport, however, recent studies have shown that HDL can prevent the oxidation of LDL . This prevention of LDL oxidation by HDL contributes to a decrease in the formation of lipid peroxides, foam cell formation and cytotoxicity otherwise caused by oLDL [12–14]. Recent evidence suggests that HDL associated enzymes such as paroxonase, may play a critical role in this protective effect [15, 16]. However, HDL itself can get oxidized and the ability of oxidized HDL (oHDL) in reverse cholesterol transport is impaired . Furthermore, oHDL is neurotoxic and has been postulated to play a role in the genesis of coronary artery spasm that contributes to the process of CHD [8, 18].
Epidemiological studies have indicated that premenopausal women have a decreased risk for the development of atherosclerosis when compared to that of age-matched males, however, this decreased risk diminishes following menopause . It has therefore been suggested that ovarian hormones, such as estrogen, play a role in the decreased risk observed in premenopausal women. During menopause, plasma LDL and HDL levels increase and decrease respectively, however studies have shown that estrogen replacement therapy (ERT) and hormone replacement therapy (HRT, estrogen plus progestin) alter these levels [20, 21], in that serum total cholesterol and LDL cholesterol decreases while HDL cholesterol increases . Until recently the modification of the plasma LDL:HDL ratio was thought to be the main mechanism for the cardioprotective effects of estrogen observed, however, recent evidence indicates that these play a minor role . More recently, it has been observed that various equine estrogens can also differentially inhibit the oxidation of the LDL and can attenuate the cytotoxicity of oLDL on neuronal cells [8, 24].
Oxidation of HDL in the presence and absence of various estrogens
The Δ6–8 ring B unsaturated equine estrogens, Eqn, 17β-Eqn and 17α-Eqn, were found to be the most potent inhibitors of HDL oxidation having two- to four-fold higher activity than 17β-E2. Similarly, Δ8-E1 and Δ8,17β-E2 were found to have 150% greater antioxidant activity than control. 17α-Eq and 17β-Eq were found to be equipotent with 17β-E2, while Eq, E1 and 17α-E2 were found to be less potent, with activities ranging from approximately 60 to 70% that of 17β-E2 (Figure 3).
Dose of Estrogen Required to Double the Lag Phase of HDL oxidation
Dose of Estrogen (nM)
LDL oxidation in the presence of HDL alone and in the presence of HDL with estrogen
In the present study, we assessed the rate of oxidation of lipoproteins by monitoring continuously the formation of conjugated dienes. The oxidation lag time is a measure of the initial resistance of lipoproteins to oxidation, in our case HDL, and has originally been used for LDL oxidation . The kinetics of HDL oxidation have been previously shown to be mechanistically similar to LDL oxidation .
A previous ex vivo study by Meng  and colleagues has shown that the incorporation of very high concentrations of esterified estrogen into HDL can prolong the oxidation lag time of HDL, whether the concentrations of estrogens incorporated inside HDL necessary for protection can be attained in vivo, remains to be determined. In our present study, we found that oHDL formation can be inhibited by all of the 10 known components of CEE and Δ8,17β-E2, an in vivo metabolite of Δ8-E1 . In contrast to the estrogen incorporation study by Meng et al , we have found that in our system antioxidant protection is possible with estrogen concentrations as low as 31 nM. A previous pharmacokinetic study  has shown that circulating estrogen levels following a single oral dose of CEE (2 × 0.625 mg) are > 70 ng/hr/mL, and thus daily pharmacological doses of CEE during ERT or HRT can provide sufficient plasma estrogen levels needed to prevent the oxidation of HDL to the extent we have observed in our in vitro study. Under our experimental conditions, esterification of estrogens was not possible, indicating that inhibition of HDL oxidation by estrogens does not require prior esterification of estrogens.
We have observed that estrogens differentially inhibit HDL oxidation. For example, Eq, 17β-Eq, and 17α-Eq all displayed antioxidant activities similar to 17β-E2, whereas the Δ6–8 and Δ8-estrogens had two- to four-fold greater activities. This difference is most likely due to the extra double bonds in the B ring, thus making the molecule more labile and therefore able to scavenge any free radicals produced more readily. For example, the ring B unsaturated estrogens were more potent antioxidants than the classical estrogens tested. Previous reports have demonstrated that the free radical scavenging activity of estrogen depends upon the phenolic structure of the molecule [30–32]. In addition to this, our observations indicate that the presence of a β-napthol ring, such as that in the Δ6–8 estrogens, may play a larger role in the antioxidant potential observed in these types of estrogens as they were found to be the most potent antioxidants. Thus, our results further extend the observation that the presence of the phenolic A ring and the 3-hydroxyl group is required for steroid antioxidant activity, but unsaturation in the B ring of the steroid moiety greatly enhances estrogens' antioxidant potency.
The order of oxidative protection for estrogens tested does not appear to involve estrogen receptors as the relative binding affinities to estrogen receptor (ER) α and β do not correlate with their antioxidant activity. For instance, compared to 17β-E2, the relative binding affinities for the ring B unsaturated estrogens: Eq, Eqn, Δ8-E1 and 17α-Eqn are 2 to 8 times lower for both ERs , whereas these same estrogens with the exception of Eq displayed the highest antioxidant potentials. Moreover, this order of antioxidant activity does not correlate with their uterotropic activities as reported earlier [24, 28, 34].
In the current study we have demonstrated that HDL can prolong the oxidation lag time of LDL using the conjugated diene formation method. This increase in oxidation lag time is most likely due to the 10-fold higher concentration of HDL over LDL as lower concentrations of HDL did not provide any protection (data not shown). Similarly, a study by Huang et al  showed HDL protection of LDL is concentration dependant. One possible mechanism for this protective effect is that HDL is preferentially oxidized over LDL and can act as a sink for harmful lipid peroxides [14, 36]. However, a more current hypothesis is that HDL associated enzymes such as paroxonase, and even the apolipoprotein Apo-A1, provide HDL with its antioxidant activity toward LDL oxidation. In support of this hypothesis, it has been shown that incubation of LDL with purified paroxonase inhibits lipid peroxidation [16, 37]. Furthermore, HDL apolipoproteins have also been demonstrated to inhibit oxidative modifications of LDL . Equine estrogens have recently been shown to stimulate the expression of Apo-A1 mRNA and protein levels by an estrogen receptor independent mechanism. Moreover, in these studies Eqn was also the most potent stimulator of Apo-A1 gene expression 
In addition to the protective effect of HDL on LDL oxidation, we have also observed that the prolongation of LDL oxidation with HDL can be enhanced with the addition of estrogen. Treatment with 17β-E2 plus HDL was more protective than 17β-E2 or HDL alone against LDL oxidation. Similarly, treatment with Eqn plus HDL displayed a greater protection against LDL oxidation than either of the constituents alone. In line with the previously defined order of protection, the Eqn plus HDL combination was observed to have a greater antioxidant effect than the 17β-E2 plus HDL combination against LDL oxidation. Also, the increased protection by the estrogen plus HDL treatment on LDL oxidation is not simply the sum of lag times with HDL or estrogen alone on LDL oxidation, and thus is suggestive of an interaction between estrogen and HDL. This estrogen enhancement of HDL's antioxidant activity is to our knowledge the first reported interaction of estrogen with HDL that causes a delay or inhibition of LDL oxidation. Recently it has been observed that HDL3 is a more effective antioxidant than HDL2 . Following ERT, the main HDL subfraction that is found to increase, is HDL2 . More studies are needed to determine the role of each HDL subfraction in presence of estrogen on the inhibition of LDL oxidation.
Although the mechanisms involved in the antioxidant properties of estrogen have not been fully defined, a number of hypotheses have been put forth. Estrogens acting as free radical scavengers are able to break the free radical chain formation produced from membrane oxidation processes and hence inhibit lipid peroxidation. In support of this, it has been postulated that estrogen may be able to sequester metal ions or donate a proton to reduce peroxy-free radicals . Furthermore, the stimulation of Apo-A1 by estrogen may also be of importance . Thus acting as antioxidants directly and by the up-regulation of antioxidant factors may be two additional mechanisms that may be involved in the cardio- and neuroprotective effects of estrogen.
In conclusion, these results indicate that HDL oxidation can be differentially inhibited by equine estrogens with the three Δ6–8 ring B unsaturated equine estrogens (Figure 1, Figure 3) being three to four times more potent than 17β-E2. Furthermore, the protection of LDL oxidation by HDL is enhanced in the presence of 17β-E2 and more so by Eqn. The inhibition of HDL oxidation and the enhancement provided by estrogen toward the inhibition of LDL oxidation may therefore be another mechanism by which estrogens reduce the risk of CHD and neurodegenerative diseases in healthy young postmenopausal women.
Materials and Methods
This study has been approved by the Research Ethics Board of St. Michael's Hospital and the University of Toronto. Blood (10 mL) was drawn by venipuncture into vacutainer tubes containing ethylenediaminetetraacetic acid (EDTA; 1 mg/mL) from fasting (overnight), postmenopausal women and healthy men. None of the subjects used were on any hormone therapy. The plasma was stabilized by addition of 6 mg/ml sucrose and either used immediately for lipoprotein isolation or stored at -80C for up to four weeks as described previously . HDL (d = 1.063-1.21 g/mL) and LDL (d = 1.019-1.063 g/mL) were isolated simultaneously by a three gradient, single-step ultracentrifugation as described previously . Following ultracentrifugation the lipoprotein containing fractions (yellow-orange/colour) were collected by aspiration. Crude HDL was further purified by re-ultracentrifugation. Assessment of absorbance at 200 to 300 nm of the crude and purified preparations of HDL, indicated that the crude preparation was essentially similar to the purified preparation and thus the single step method of isolation was used for the experiments described. The LDL isolated by this method had properties similar to those previously described . The freshly isolated lipoproteins were stored at 4C under argon and used within 1 week of preparation. Prior to oxidation, EDTA was removed from the lipoprotein isolate by rapid gel filtration through disposable desalting columns (ECONO-PAC 10 DG, Bio-Rad Laboratories). The concentration of protein in each lipoprotein isolate was measured using the Bradford method (Bio-Rad Protein Assay kit, catalog number 500–0006, Mississauga, ON, Canada) .
HDL oxidation in the presence and absence of various concentrations of estrogens
Oxidation of 50 μg HDL was induced by addition of 1.67 μM CuSO4 in the presence or absence of 0.025 μg, 0.05 μg, 0.25 μg, 0.5 μg, 1 μg of the 11 estrogens. The kinetics of lipoprotein oxidation were assessed by measuring the rate of formation of conjugated dienes measured at 234 nm over a period of 6 hours at 32C. Absorbance was measured in a Beckman DU 640 spectrophotometer equipped with a six-position cuvette changer and a Peltier temperature controller (Beckman Instruments, Mississauga, ON, Canada) essentially as previously described [8, 25]. The extent of lipoprotein oxidation was confirmed by assessing the electrophoretic mobility of oHDL and oLDL on a 1% agarose gel in 0.1 M Tris, pH 8.6 compared to the native form of HDL and LDL [3, 8, 45, 46].
LDL oxidation in the presence of HDL and estrogen plus HDL
Oxidation of 20 μg LDL was induced in a similar manner, however, in this case the effect of 200 μg HDL and the combination of 17β-E2 plus 200 μg HDL or Eqn plus 200 μg HDL on LDL oxidation were assessed.
Analysis of data
The oxidation lag phase, defined as the point at which the initial slope representing the initiation rate and the point at which the propagation slope intersect, was calculated from the kinetic plot of the change in absorbance versus time as described previously [8, 25, 42]. From the dose response curves, the concentration of each estrogen (nM) required to double the length of the control lag time of oxidation, was calculated. For comparison purposes, all values obtained were adjusted to percent activity of 17β-E2.
This work was supported by the medical research council of Canada grant MT-11929 and a basic research grant from Women's Health Care Research, Wyeth Pharmaceuticals, Philadelphia, PA, USA. We wish to thank Ms. Julie Binkley for assisting in the lipoprotein preparations.
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