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Table 2 Incorporation of 5,11,14 20:3 into cultured keratinocyte phospholipids

From: Epidermal anti-Inflammatory properties of 5,11,14 20:3: Effects on mouse ear edema, PGE2 levels in cultured keratinocytes, and PPAR activation

Treatment with fatty acids

Fatty acids incorporated into total phospholipids (%)

Exp #

Day 0

Day 2

Day 4

5,11,14 20:3

20:3n-6

20:4n-6

20:5n-3

1

-

EtOH

EtOH

0.00

0.11

0.43

0.04

2

-

5,11,14 20:3

5,11,14 20:3

3.34

0.17

0.47

0.06

3

 

20:4n-6

20:4n-6

0.30

0.55

10.39

0.00

4

20:4n-6

5,11,14 20:3

5,11,14 20:3

2.12

0.38

5.30

0.03

5

20:4n-6

20:4n-6

20:4n-6

0.09

0.85

11.80

0.03

  1. Human DK2-NR keratinocytes were incubated in NR-2 medium containing 15 μM concentrations of various FA methyl esters for up to 6 d as indicated above. Exp, Experiment. Values represent mean of 3 replicates.