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Table 2 Incorporation of 5,11,14 20:3 into cultured keratinocyte phospholipids

From: Epidermal anti-Inflammatory properties of 5,11,14 20:3: Effects on mouse ear edema, PGE2 levels in cultured keratinocytes, and PPAR activation

Treatment with fatty acids Fatty acids incorporated into total phospholipids (%)
Exp # Day 0 Day 2 Day 4 5,11,14 20:3 20:3n-6 20:4n-6 20:5n-3
1 - EtOH EtOH 0.00 0.11 0.43 0.04
2 - 5,11,14 20:3 5,11,14 20:3 3.34 0.17 0.47 0.06
3   20:4n-6 20:4n-6 0.30 0.55 10.39 0.00
4 20:4n-6 5,11,14 20:3 5,11,14 20:3 2.12 0.38 5.30 0.03
5 20:4n-6 20:4n-6 20:4n-6 0.09 0.85 11.80 0.03
  1. Human DK2-NR keratinocytes were incubated in NR-2 medium containing 15 μM concentrations of various FA methyl esters for up to 6 d as indicated above. Exp, Experiment. Values represent mean of 3 replicates.