A new chymotrypsin-like serine protease involved in dietary protein digestion in a primitive animal, Scorpio maurus: purification and biochemical characterization

Table 3 Specific activity of scorpion protease (U/mg) measured on various substrates.

Substrate	TAME^a	BAPNA^b	SAPNA^c	Casein
Scorpion protease	< 0.001	0.01 ± 0.002	35 ± 0.65	75000 ± 5400
Bovine chymotrypsin protease	nd	nd	280 ± 1.34	197080 ± 8750
Porcine trypsin protease	1.3 ± 0.12	4.5 ± 0.4	nd	59125 ± 4800

Bovine chymotrypsin (BCP) and porcine trypsin (PTP) were taken as positive standards for comparative purposes.
Data are expressed as means ± SD of three determinations.
^aTAME: N alpha-(p-toluene sulfonyl)-L-arginine methyl ester;^bBAPNA: benzoyl-Arginine-p-nitroanilide; ^cSAPNA: N-Succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide; nd: not determined.

ISSN: 1476-511X