Skip to main content

Advertisement

Table 3 Specific activity of scorpion protease (U/mg) measured on various substrates.

From: A new chymotrypsin-like serine protease involved in dietary protein digestion in a primitive animal, Scorpio maurus: purification and biochemical characterization

Substrate TAMEa BAPNAb SAPNAc Casein
Scorpion protease < 0.001 0.01 ± 0.002 35 ± 0.65 75000 ± 5400
Bovine chymotrypsin protease nd nd 280 ± 1.34 197080 ± 8750
Porcine trypsin protease 1.3 ± 0.12 4.5 ± 0.4 nd 59125 ± 4800
  1. Bovine chymotrypsin (BCP) and porcine trypsin (PTP) were taken as positive standards for comparative purposes.
  2. Data are expressed as means ± SD of three determinations.
  3. aTAME: N alpha-(p-toluene sulfonyl)-L-arginine methyl ester;bBAPNA: benzoyl-Arginine-p-nitroanilide; cSAPNA: N-Succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide; nd: not determined.