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Table 2 Effects of different metal ions on the activity of stingray PLA2

From: Proteolytic cleavage of stingray phospholipase A2: Isolation and biochemical characterization of an active N-terminal form

Ions

PLA2 Activity (%)

 

0 mM Ca2+

1 mM Ca2+

Mg 2+

3.5 ± 1.1

60 ± 5.4

Mn 2+

2 ± 1

57 ± 2.2

Zn 2+

3 ± 0.5

15 ± 2.4

Cd 2+

2 ± 0.8

12 ± 1.8

  1. Influence of ions concentrations (10 mM each) on SPLA2 activity in the absence or presence of 1 mM Ca2+ was studied using PC emulsion as substrate at 40°C and pH8.5. The control represents 100% of PLA2 activity at 8 mM Ca2+ under the same condition. Values represent the mean of three replicates.
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Lipids in Health and Disease

ISSN: 1476-511X