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Table 2 Fatty acid distribution of CLA isomers in different milk lipids

From: Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

  A B
  MLcon   MLalp   MLcon   MLalp  
CLA FAME [%] Mean   SD   Mean   SD   Mean   SD   Mean   SD  
t 12,t 14 2.36 ± 0.08 a 0.96 ± 0.01 b 2.34 ± 0.16 a 1.00 ± 0.02 b
t 11,t 13 4.19 ± 0.09 a 1.86 ± 0.05 b 4.05 ± 0.16 a 1.92 ± 0.06 b
t 10,t 12 0.95 ± 0.07 a 0.17 ± 0.04 b 0.97 ± 0.09 a 0.33 ± 0.09 b
t 9,t 11 2.68 ± 0.24 b 0.54 ± 0.03 c 3.27 ± 0.16 a 0.85 ± 0.20 c
t 8,t 10 1.41 ± 0.08 a 0.19 ± 0.03 b 1.53 ± 0.21 a 0.22 ± 0.09 b
t 7,t 9 0.42 ± 0.03 a 0.07 ± 0.03 b 0.41 ± 0.10 a 0.06 ± 0.03 b
trans,trans 12.18 ± 0.24 a 3.84 ± 0.07 b 12.69 ± 0.74 a 4.42 ± 0.44 b
t 11,c 13 1.51 ± 0.15 b 7.88 ± 0.02 a 1.29 ± 0.08 b 7.83 ± 0.09 a
c 11,t 13 0.39 ± 0.14   0.17 ± 0.03   0.25 ± 0.12   0.24 ± 0.07  
t 10,c 12 0.51 ± 0.03 ab 0.07 ± 0.04 b 0.74 ± 0.26 a 0.32 ± 0.38 ab
c 9,t 11 74.46 ± 0.59 b 84.57 ± 0.19 a 74.64 ± 0.63 b 83.77 ± 0.86 a
t 8,c 10 0.91 ± 0.25   0.60 ± 0.13   1.21 ± 0.28   0.61 ± 0.27  
t 7,c 9 8.97 ± 0.28 a 2.64 ± 0.23 b 8.39 ± 0.09 a 2.56 ± 0.34 b
cis,trans/trans,cis 86.90 ± 0.25 b 95.99 ± 0.02 a 86.62 ± 0.75 b 95.39 ± 0.42 a
c 11,c 13 0.07 ± 0.04   0.10 ± 0.06   0.10 ± 0.06   0.09 ± 0.03  
c 10,c 12 0.10 ± 0.08   -   0.05 ± 0.01   -  
c 9,c 11 0.67 ± 0.20 a 0.07 ± 0.01 c 0.54 ± 0.30 ab 0.10 ± 0.01 ab
c 8,c 10 -   -   -   -  
cis/cis 0.84 ± 0.08 a 0.17 ± 0.06 b 0.69 ± 0.33 a 0.18 ± 0.02 b
t 11,c 13/t 7,c 9 0.17 ± 0.02 b 3.00 ± 0.26 a 0.15 ± 0.01 b 3.10 ± 0.48 a
  1. FA distribution of milk lipids from TAG (ML conventional and ML Alpine) before preparation (A) to FFA-mixtures suitable for cellular experiments, FA distribution of milk lipids as FFA-mixtures used for incubation of cells after saponification, extraction and solution (B) of milk lipids (FFA) in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter (a, b, c, d), two-way ANOVA Tukey post hoc test P < 0.05.