Lipids in Health and Disease

Table 2 Fatty acid distribution of CLA isomers in different milk lipids

From: Influence of in vitro supplementation with lipids from conventional and Alpine milk on fatty acid distribution and cell growth of HT-29 cells

	A								B
	ML_con				ML_alp				ML_con				ML_alp
CLA FAME [%]	Mean		SD		Mean		SD		Mean		SD		Mean		SD
t 12,t 14	2.36	±	0.08	^a	0.96	±	0.01	^b	2.34	±	0.16	^a	1.00	±	0.02	^b
t 11,t 13	4.19	±	0.09	^a	1.86	±	0.05	^b	4.05	±	0.16	^a	1.92	±	0.06	^b
t 10,t 12	0.95	±	0.07	^a	0.17	±	0.04	^b	0.97	±	0.09	^a	0.33	±	0.09	^b
t 9,t 11	2.68	±	0.24	^b	0.54	±	0.03	^c	3.27	±	0.16	^a	0.85	±	0.20	^c
t 8,t 10	1.41	±	0.08	^a	0.19	±	0.03	^b	1.53	±	0.21	^a	0.22	±	0.09	^b
t 7,t 9	0.42	±	0.03	^a	0.07	±	0.03	^b	0.41	±	0.10	^a	0.06	±	0.03	^b
∑ trans,trans	12.18	±	0.24	^a	3.84	±	0.07	^b	12.69	±	0.74	^a	4.42	±	0.44	^b
t 11,c 13	1.51	±	0.15	^b	7.88	±	0.02	^a	1.29	±	0.08	^b	7.83	±	0.09	^a
c 11,t 13	0.39	±	0.14		0.17	±	0.03		0.25	±	0.12		0.24	±	0.07
t 10,c 12	0.51	±	0.03	^ab	0.07	±	0.04	^b	0.74	±	0.26	^a	0.32	±	0.38	^ab
c 9,t 11	74.46	±	0.59	^b	84.57	±	0.19	^a	74.64	±	0.63	^b	83.77	±	0.86	^a
t 8,c 10	0.91	±	0.25		0.60	±	0.13		1.21	±	0.28		0.61	±	0.27
t 7,c 9	8.97	±	0.28	^a	2.64	±	0.23	^b	8.39	±	0.09	^a	2.56	±	0.34	^b
∑ cis,trans/trans,cis	86.90	±	0.25	^b	95.99	±	0.02	^a	86.62	±	0.75	^b	95.39	±	0.42	^a
c 11,c 13	0.07	±	0.04		0.10	±	0.06		0.10	±	0.06		0.09	±	0.03
c 10,c 12	0.10	±	0.08		-				0.05	±	0.01		-
c 9,c 11	0.67	±	0.20	^a	0.07	±	0.01	^c	0.54	±	0.30	^ab	0.10	±	0.01	^ab
c 8,c 10	-				-				-				-
∑ cis/cis	0.84	±	0.08	^a	0.17	±	0.06	^b	0.69	±	0.33	^a	0.18	±	0.02	^b
t 11,c 13/t 7,c 9	0.17	±	0.02	^b	3.00	±	0.26	^a	0.15	±	0.01	^b	3.10	±	0.48	^a

FA distribution of milk lipids from TAG (ML conventional and ML Alpine) before preparation (A) to FFA-mixtures suitable for cellular experiments, FA distribution of milk lipids as FFA-mixtures used for incubation of cells after saponification, extraction and solution (B) of milk lipids (FFA) in ethanol. Means (n = 6) of FAME [%] significantly differ without the same letter ^{(a, b, c, d)}, two-way ANOVA Tukey post hoc test P < 0.05.

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