Accumulation and aberrant composition of cholesteryl esters in Scrapie-infected N2a cells and C57BL/6 mouse brains

Table 2 Amplex Red vs. HPLC-MS cholesterol compositions in brains of uninfected and Scrapie-infected C57BL/6 mice.

Method	Mouse brains	μ mol/g tissue			CE/TC %
		TC	FC	CE
Amplex Red	Uninfected	276.38 ± 11.78	275.24 ± 14.44	1.14 ± 1.24	0.41
	Scrapie-infected	283.48 ± 8.74	282.24 ± 9.88	1.24 ± 1.59	0.43
		p = 0.351	p = 0.438	p = 0.918
HPLC-MS	Uninfected	124.35 ± 29.61	112.87 ± 17.37	11.48 ± 3.26	9.23
	Scrapie-infected	156.58 ± 25.46	137.24 ± 16.70	19.33 ± 3.20*	12.34
		p = 0.129	p = 0.071	*p = 0.008*

Total (TC), free (FC) and esterified (CE) cholesterol in brain extracts of uninfected (n = 5) and 139A-scrapie infected (n = 4) mice. Values represent the mean ± SD of triplicate determinations and are representative of at least three independent trials. *Statistical significance by unpaired Student's t-test.

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