Skip to main content

Table 2 Amplex Red vs. HPLC-MS cholesterol compositions in brains of uninfected and Scrapie-infected C57BL/6 mice.

From: Accumulation and aberrant composition of cholesteryl esters in Scrapie-infected N2a cells and C57BL/6 mouse brains

Method Mouse brains μ mol/g tissue CE/TC %
   TC FC CE  
Amplex Red Uninfected 276.38 ± 11.78 275.24 ± 14.44 1.14 ± 1.24 0.41
  Scrapie-infected 283.48 ± 8.74 282.24 ± 9.88 1.24 ± 1.59 0.43
   p = 0.351 p = 0.438 p = 0.918  
HPLC-MS Uninfected 124.35 ± 29.61 112.87 ± 17.37 11.48 ± 3.26 9.23
  Scrapie-infected 156.58 ± 25.46 137.24 ± 16.70 19.33 ± 3.20* 12.34
   p = 0.129 p = 0.071 p = 0.008  
  1. Total (TC), free (FC) and esterified (CE) cholesterol in brain extracts of uninfected (n = 5) and 139A-scrapie infected (n = 4) mice. Values represent the mean ± SD of triplicate determinations and are representative of at least three independent trials. *Statistical significance by unpaired Student's t-test.