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Table 3 Amplex Red vs. HPLC-MS cholesterol compositions in brains of untreated and pravastatin-treated Scrapie-infected C57BL/6 mice.

From: Accumulation and aberrant composition of cholesteryl esters in Scrapie-infected N2a cells and C57BL/6 mouse brains

Method Scrapie-infected mouse brains μ mol/g tissue CE/TC %
   TC FC CE  
Amplex Red Untreated 283.48 ± 8.74 282.24 ± 9.88 1.24 ± 1.59 0.43
  PRV-treated 279.12 ± 6.84 276.68 ± 8.74 2.44 ± 1.27 0.87
   p = 0.462 p = 0.432 p = 0.283  
HPLC-MS Untreated 156.58 ± 25.46 137.24 ± 16.70 19.33 ± 3.20 12.34
  PRV-treated 134.17 ± 27.74 106.03 ± 13.46* 28.14 ± 6.62* 20.97
   p = 0.247 p = 0.026 p = 0.05  
  1. Total (TC), free (FC) and esterified (CE) cholesterol in brain extracts of 139A-scrapie infected (n = 4), and of 139A-scrapie-infected C57Bl/6 mice treated with pravastatin (PRV, 200 mg/kg b.w.). Values represent the mean ± SD of triplicate determinations and are representative of at least three independent. *Statistical significance by unpaired Student's t-test.