Table 1 Gene expression results
Gene Symbol | Affymetrix ID | Refseq NCBI ID | Transcript name | SW620 Fold Change |
|---|---|---|---|---|
| Â | Â | Â | Â | 24 h |
ER stress and unfolded protein response | Â | Â | ||
ATF3 | 202672_s_at | NM_001030287 NM_001040619 NM_001674 NM_004024 | Activating transcription factor 3 | 3.5 |
ATF4 | 200779_at | NM_001675 NM_182810 | Activating transcription factor 4 | 1.7 |
ATF6 | 217550_at 231927_at 203952_at | NM_007348 | Activating transcription factor 6 | 1.5 1.3 1.2 |
CEBPB | 212501_at | NM_005194 | CCAAT/enhancer binding protein (C/EBP), beta | 3.2 |
CHOP/DDIT3 | 209383_at | NM_001130101 NM_001130102 NM_004083 NM_005693 | DNA-damage-inducible transcript 3 | 2.7 |
GADD34 | 37028_at 202014_at | NM_014330 | Growth arrest and DNA-damage-inducible 34 | 2.0 2.0 |
IRE1/ERN1 | 235745_at | NM_001433 | Inositol-requiring enzyme 1/Endoplasmic reticulum to nucleus signaling 1 | 1.7 |
NRF2/NFE2L2 | 201146_at | NM_001145412 NM_001145413 NM_006164 | Nuclear factor (erythroid-derived 2)-like 2 | 1.3 |
TRIB3 | 1555788_a_at 218145_at | NM_021158 | Tribbles homolog 3 (Drosophila) | 5.1 4.9 |
XBP1 | 242021_at 200670_at | NM_001079539 NM_005080 | X-box binding protein 1 | 1.2 1.6 |
Chaperones/Protein folding/Unfolded protein response/Protein degradation/Amino acid synthesis | Â | |||
ASNS | 205047_s_at | NM_001673 NM_133436 NM_183356 | Asparagine synthetase | 4.8 |
CREB3L2 | 212345_s_at | NM_194071 | cAMP responsive element binding protein 3-like 2 | 1.4 |
CREB3L3 | 234361_at | NM_032607 | cAMP responsive element binding protein 3-like 3 | 3.0 |
DNAJB2 | 202500_at | NM_001039550 NM_006736 | DnaJ (Hsp40) homolog, subfamily B, member B2 | 1.3 |
DNAJB14 | 222850_s_at | NM_001031723 | DnaJ (Hsp40) homolog, subfamily B, member B14 | 1.2 |
DNAJC24 | 242562_at | NM_181706 | DnaJ (Hsp40) homolog, subfamily C, member 24 | 1.4 |
EDEM1 | 203279_at | NM_014674 | ER degradation enhancer, mannosidase alpha-like 1 | 1.3 |
EDEM3 | 220342_x_at | NM_025191 | ER degradation enhancer, mannosidase alpha-like 3 | 1.2 |
ERO1LB | 231944_at | NM_019891 | ERO1-like beta (S. cerevisiae) | 1.3 |
HMOX1/HSP32 | 203665_at | NM_002133 | Heme oxygenase (decycling) 1 | 1.6 |
HSPA13 | 202557_at 202558_s_at | NM_006948 | Heat shock 70kDa protein 13 | 1.4 1.7 |
PDIA2 | 206691_s_at | NM_006849 | Protein disulfide isomerase family A, member 2 | 1.2 |
PFDN2 | 218336_at | NM_012394 | Prefoldin 2 | 1.4 |
PSMB1 | 214289_at | NM_002793 | Proteasome subunit, beta type, 1 | 1.3 |
SQSTM1 | 201471_s_at 213112_s_at 239004_at 244804_at | NM_001142298 NM_001142299 NM_003900 | Sequestosome 1 | 2.1 3.1 1.3 2.1 |
UBE2B | 239163_at | NM_003337 | Ubiquitin-conjugating enzyme E2B (RAD6 homolog) | 1.5 |
SEC61A2 | 219499_at 228747_at | NM_001142627 NM_001142628 NM_018144 | Sec61 alpha 2 subunit (S. cerevisiae) | 1.3 1.2 |
SEC61B | 244700_at | NM_006808 | Sec61 beta subunit | 1.6 |
SEC63 | 201914_s_at 201915_s_at 201916_s_at | NM_007214 | SEC63 homolog (S. cerevisiae) | 1.3 1.2 1.3 |
Ca2 + homeostasis, signaling and transport | Â | |||
ATP2B4/PMCA4 | 205410_s_at 212135_s_at 212136_at | NM_001001396 NM_001684 | ATPase, Ca2+ transporting, plasma membrane 4 | 1.5 2.7 5.4 |
CAPN1 | 232012_at | NM_005186 | Calpain 1, (mu/I) large subunit | 1.4 |
CAPN2 | 208683_at 214888_at | NM_001146068 NM_001748 | Calpain 2, large subunit | 1.4 1.8 |
CAPN5 | 205166_at | NM_004055 | Calpain 5 | 1.4 |
HERPUD1 | 217168_s_at | NM_001010989 NM_001010990 NM_014685 | Homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 | 1.9 |
ITPR1 | 244090_at | --- | Inositol 1,4,5-triphosphate receptor, type 1 | 1.3 |
ITPR3 | 201187_s_at 201188_s_at 201189_s_at | NM_002224 | Inositol 1,4,5-triphosphate receptor, type 3 | 1.3 1.5 1.5 |
PLCD3 | 234971_x_at 1552476_s_at | NM_133373 | Phospholipase C, delta 3 | 1.3 1.3 |
S100A10 | 200872_at 238909_at | NM_002966 | S100 calcium binding protein A10 | 1.2 2.4 |
S100P | 204351_at | NM_005980 | S100 calcium binding protein P | 4.9 |
STC2 | 203438_at 203439_s_at | NM_003714 | Stanniocalcin 2 | 2.1 2.7 |
Cell cycle/Apoptosis | Â | Â | Â | Â |
ATF5 | 204998_s_at 204999_s_at | NM_012068 | Activating transcription factor 5 | 1.5 1.7 |
AURKA | 208080_at | NM_003600 NM_198433 NM_198434 NM_198435 NM_198436 NM_198437 | Aurora kinase A | -1.3 |
BIRC5 | 202094_at 202095_s_at 210334_x_at | NM_001012270 NM_001012271 NM_001168 | Baculoviral IAP repeat-containing 5/Survivin | -1.7 -1.3 -1.3 |
CASP4 | 213596_at | NM_001225 NM_033306 | Caspase 4 | 1.4 |
CCNA2 | 203418_at 213226_at | NM_001237 | Cyclin A2 | -1.2 -1.2 |
CCND3 | 201700_at | NM_001136017 NM_001136125 NM_001136126 NM_001760 | Cyclin D3 | -2.0 |
CCNE1 | 213523_at | NM_001238 NM_057182 | Cyclin E1 | -1.3 |
CCNE2 | 205034_at 211814_s_at | NM_057749 | Cyclin E2 | -2.9 -3.1 |
CCNF | 204827_s_at | NM_001761 | Cyclin F | -1.2 |
CDC2/CDK1 | 203214_x_at | NM_001130829 NM_001786 NM_033379 | Cell division cycle 2, G1 to S and G2 to M | -1.2 |
CDK2 | 204252_at 211804_s_at | NM_001798 NM_052827 | Cyclin-dependent kinase 2 | -1.3 -1.4 |
CDK4 | 202246_s_at | NM_000075 | Cyclin-dependent kinase 4 | -1.4 |
CDK5 | 204247_s_at | NM_001164410 NM_004935 | Cyclin-dependent kinase 5 | -1.5 |
CDK6 | 224847_at 224848_at 224851_at 243000_at | NM_001145306 NM_001259 | Cyclin-dependent kinase 6 | -1.4 -1.5 -1.3 -1.3 |
CDKN2A | 211156_at | NM_000077 NM_058195 NM_058197 | Cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) | 1.3 |
CDKN2B | 207530_s_at 236313_at | NM_004936 NM_078487 | Cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) | 1.5 2.0 |
CDKN2D | 210240_s_at | NM_001800 NM_079421 | Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) | 1.2 |
CUL1 | 238509_at | NM_003592 | Cullin 1 | 1.4 |
KLF4 | 220266_s_at 221841_s_at | NM_004235 | Kruppel-like factor 4 (gut) | 1.9 2.4 |
PDCD4 | 202731_at 212593_s_at | NM_014456 NM_145341 | Programmed cell death 4 (neoplastic transformation inhibitor) | 1.3 1.3 |
PDCD6 | 222152_at 222380_s_at | NM_013232 | Programmed cell death 6 | 1.3 1.3 |
SFN | 33322_i_at 33323_r_at 209260_at | NM_006142 | Stratifin | 1.6 1.7 1.6 |