Skip to main content
Figure 5 | Lipids in Health and Disease

Figure 5

From: Immunohistochemical localization of hepatopancreatic phospholipase in gastropods mollusc, Littorina littorea and Buccinum undatum digestive cells

Figure 5

Immunohistochemistry and immunofluorescence localization of phospholipase in a 4-μm frozen sections. (A) Sections of digestive glands showing mSDPLA2 immunoreactivity in digestive tubules. Few mSDPLA2 positive nuclei appeared. These immunoreactive nuclei corresponded mainly to secretory cells (arrows), whereas the nucleus of digestive cells was mSDPLA2 negative (B). (B) Control section of digestive gland incubated in absence of anti- mSDPLA2 antiserum. Any reaction was disclosed. (C) One section was first counter-stained with DAPI for observing general morphology. (D and E) followed by pAbs anti-mSDPL antiserum (1:100) revealed by fluorochrome (chromeo™488) conjugated to the secondary antibody diluted 1:200 in PBS.

Back to article page

Lipids in Health and Disease

ISSN: 1476-511X