Figure 1

M2 polarisation and apoptosis/cell viability in primary macrophage cell types. A: RT-PCR was used to determine relative mRNA expression levels for the M2 markers MR (white bars) and IL-Ra (grey bars) before ('hMΦ') and after ('hM2MΦ') treatment of human primary macrophages with 15 ng/mL IL-13/1 μM Rosiglitazone (72 h). n ≥ 3 in all cases; P < 0.10 where indicated. B: RT-PCR was used to determine relative mRNA expression levels for the M2 markers MR (white bars) and IL-Ra (grey bars) in THP1-Mon (white bars), THP1-M2Mon (light grey bars), dTHP1-MΦ (dark grey bars) and dTHP1-M2MΦ (black bars) n ≥ 3 in all cases; P < 0.05 where indicated. Spectrophotometric MTS assays (C) and luminometric Caspase 3/7 assays (D) were used to determine cell viability and apoptosis respectively in hMΦ (white bars) and hM2MΦ (black bars), after treatment with oxLDL (1 μg/ml or 40 μg/ml; 24 h) as indicated. n ≥ 3 in all cases.