Figure 7
Effect of increasing concentrations of bile salt (NaDC) and calcium ions (Ca2+) on the rate of hydrolysis of olive oil emulsion by the rTPL. (A) Effect of increasing concentration of bile salt (NaDC) on the rate of hydrolysis of olive oil emulsion by the rTPL and the nTPL. Lipolytic activity was measured at pH 8.5 and 37°C in the absence or in the presence of a molar excess of colipase. (B) Effect of increasing concentration of calcium ions (Ca2+) on the rate of hydrolysis of olive oil emulsion by the rTPL and the nTPL. Lipolytic activity was measured at pH 8.5 and 37°C. The star indicates the lipase activity measured in the absence of CaCl2 and in the presence of 10 mM EDTA.