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Table 1 Flow sheet of rTPL purification

From: Purification and characterization of the first recombinant bird pancreatic lipase expressed in Pichia pastoris: The turkey

Purification step Total activity
(U)
Specific activity
(U/mg)
Yield
(%)
Fold
Supernatant 34380 399 100 1
(NH4)2SO4 Precipitation 30800 1294 89.6 3.24
DEAE-Cellulose 24650 1454 71.7 3.64
Sephacryl S-200 23540 3789 68.5 9.49
Q-Sepharose FF 14790 4150 43 10.4
Sephadex G-100 11000 5300 32 13.28