Purification and characterization of the first recombinant bird pancreatic lipase expressed in Pichia pastoris: The turkey

Table 1 Flow sheet of rTPL purification

Purification step	Total activity (U)	Specific activity (U/mg)	Yield (%)	Fold
Supernatant	34380	399	100	1
(NH₄)₂SO₄ Precipitation	30800	1294	89.6	3.24
DEAE-Cellulose	24650	1454	71.7	3.64
Sephacryl S-200	23540	3789	68.5	9.49
Q-Sepharose FF	14790	4150	43	10.4
Sephadex G-100	11000	5300	32	13.28

ISSN: 1476-511X