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Table 1 Flow sheet of stingray PLA2 purification.

From: Purification and biochemical characterization of pancreatic phospholipase A2 from the common stingray Dasyatis pastinaca

Purification
step
Total (a) activity (units) Protein (b)
(mg)
Specific
activity
(U/mg)
Activity
recovery
(%)
Purification
factor
Extraction 9000 3500 2.6 100 1
Heat and acidic treatment 7650 1050 7.3 72 2.8
(NH 4 ) 2 SO 4
Precipitation
(30-70%)
6120 500 12.3 68 4.7
Ethanol fractionation
(50-90%)
5250 150 35 58 13.46
Mono-S
Sepharose
4050 8.1 250 45 96
Mono-Q
Sepharose
2900 5.3 550 32 211.5
RP-HPLC 2100 2.8 750 23 288.5
  1. (a) 1 Unit: μmole of fatty acid released per min using PC emulsion as substrate in the presence of 4 mM NaTDC and in the presence of 8 mM CaCl2.
  2. (b) Proteins were estimated by Bradford method (32). The experiments were conducted three times.