Figure 6
From: Function modification of SR-PSOX by point mutations of basic amino acids
Cells adhesion ability in the transfected 293T cells. 293T cells were transiently transfected with pEGFP-N3 empty vector, wild-type SR-PSOX and different mutant constructs respectively. After 24 hours transfection, the cells were incubated with EDTA for 10 minutes at 37°C. The number of adhesion cell was quantified by crystal violet stain. OD value was used to measure the adhesive ability on the cells. The data show the mean ± SEM from three independent experiments. *: P < 0.05, **: P < 0.01, each compared to wild-type SR-PSOX (WT).