Figure 2
From: Effects of n-3 PUFAs on breast cancer cells through their incorporation in plasma membrane
Effects of n-3 PUFA on Bcl2 expression. Both cell lines were treated with DHA (200 μM) or EPA (230 μM) for 72 h. Control and treated cell lysates are separated on 10% SDS-PAGE and transferred to PVDF membrane. The expression of the anti-apoptotic protein Bcl2 was assessed by western blot and semi-quantitative analysis performed by plate scanning. β actin was used to normalize results of protein content. A: MCF7 cells, B MDA-MB-231 cells.