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Figure 1 | Lipids in Health and Disease

Figure 1

From: 2-hydroxylated sphingomyelin profiles in cells from patients with mutated fatty acid 2-hydroxylase

Figure 1

Lymphocyte FA2H patient transcript and total FA-SM/OH-SM profiles. A, Lane C shows the transcript obtained in control lymphocytes using the FA2H primer and lane P shows the shorter transcript obtained in patient lymphocytes. B, Total level of FA-SM in control and patient fibroblasts, lymphocytes and erythrocytes was quantitated by HPLC/MS-MS. C, Total level of OHFA-SM in control and patient fibroblasts, lymphocytes and erythrocytes was quantitated by HPLC/MS-MS. D, The ratio of the total OHFA-SM content divided by the total FA-SM content was calculated (mean of 2 experiments +/- SD).

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