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Table 3 Correlations between sphingomyelinase, ceramidase and sphingosine kinase gene expression, and ceramide and sphingomyelin concentrations in subcutaneous adipose tissue

From: Expression of ceramide-metabolising enzymes in subcutaneous and intra-abdominal human adipose tissue

  SMPD1 SMPD2 SMPD3 ASAH1 SPHK1
  r P r P r P r P r P
Cer(d18:0/22:0) 0.380 0.097 −0.170 0.459 0.244 0.288 0.005 0.984 0.259 0.260
Cer(d18:1/16:0) 0.065 0.778 0.089 0.699 0.498 0.030 0.047 0.839 0.361 0.116
Cer(d18:1/24:1) 0.408 0.076 0.283 0.218 0.552 0.016 0.344 0.133 0.526 0.022
SM(d18:1/16:0) 0.135 0.555 0.020 0.932 0.211 0.359 −0.310 0.177 −0.203 0.376
SM(d18:1/18:0) 0.281 0.220 0.411 0.074 0.496 0.031 −0.164 0.475 0.215 0.349
SM(d18:1/20:0) 0.398 0.082 0.376 0.101 0.555 0.016 −0.338 0.140 0.492 0.032
SM(d18:1/22:0) 0.331 0.149 0.356 0.120 0.595 0.009 0.075 0.743 0.474 0.039
SM(d18:1/22:1) 0.415 0.070 0.290 0.206 0.582 0.011 −0.081 0.723 0.445 0.052
SM(d18:1/24:1) 0.253 0.271 0.423 0.066 0.567 0.014 0.167 0.467 0.289 0.208
SM(d18:1/24:2) 0.329 0.151 0.319 0.165 0.513 0.025 −0.033 0.885 0.405 0.078
  1. Correlations between gene expression levels of SMPD1-3 (sphingomyelinases), ASAH1 (ceramidase) and SPHK1 (sphingosine kinase) and concentrations of various ceramides (cer) and sphingomyelins (SM) in subcutaneous adipose tissue of obese women (n = 20). Spearman rank correlation coefficients and significance levels are shown. Comparison of sphingolipid concentrations (mean ± SEM, nmol/g adipose tissue) in subcutaneous adipose tissue from women with less inflamed adipose tissue/normal liver fat (n = 10) versus more inflamed adipose tissue/high liver fat content (n = 10), as reported previously [3]: Cer(d18:0/22:0): 3.80 ± 0.58 vs 6.21 ± 1.11, P = 0.076; Cer(d18:1/16:0): 2.66 ± 0.28 vs 3.12 ± 0.23, P = 0.219; Cer(d18:1/24:1): 5.36 ± 0.47 vs 7.97 ± 0.70, P = 0.006; SM(d18:1/16:0): 36.86 ± 2.64 vs 35.10 ± 2.64, P = 0.643; SM(d18:1/18:0): 4.88 ± 0.40 vs 6.02 ± 0.46, P = 0.077; SM(d18:1/20:0): 5.84 ± 0.50 vs 7.30 ± 0.52, P = 0.055; SM(d18:1/22:0): 11.38 ± 0.96 vs 15.38 ± 1.15, P = 0.015; SM(d18:1/22:1): 7.13 ± 0.54 vs 8.54 ± 0.67, P = 0.121; SM(d18:1/24:1): 17.00 ± 1.33 vs 22.96 ± 1.70, P = 0.013; SM(d18:1/24:2): 7.42 ± 0.56 vs 9.69 ± 0.76, P = 0.028. Lipidomic analysis was described previously [3].