The effect of DHT on AapoM expression is independent of the classical androgen receptor. HepG2 cells were treated with 10 μM flutamide or vehicle for 30 min and then incubated in the presence of different concentrations of DHT for 24 h. ApoM concentrations were determined by western blotting analysis (A), and ApoM mRNA levels were determined by RT-PCR (B) as described in “Materials and methods.” Data are expressed relative to the control group (100%). Data are represented as means ± S.D. (n = 6 for each sample group). Lane 1, control group, lanes 2–9, DHT concentrations of 1, 3, 10, 30, 100, 300, 1000 and 10000nM respectively. *,# P < 0.05 versus control group.