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Figure 3 | Lipids in Health and Disease

Figure 3

From: Mitochondrial function is involved in regulation of cholesterol efflux to apolipoprotein (apo)A-I from murine RAW 264.7 macrophages

Figure 3

The effects of mitochondrial inhibitors, antimycin, dinitrophenol, nigericin and oligomycin, each used at 10μM for 3 h, on efflux of [3H]cholesterol to apoA-I (20μg ml-1) is shown in Figure3A. Where indicated, macrophages were preincubated for 24 h in the presence of dibutyryl cAMP (0.3 mM) to induce expression of ABCA1 and cholesterol efflux[25]. Values are the mean ± SEM of between four to six independent experiments; **p<0.01 compared with control macrophages; other significant changes are indicated on the figure. Expression of ABCA1 protein under the conditions noted is shown in Figure3B; values are shown relative to the housekeeping gene, GAPDH, and are representative of three independent experiments.

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