Figure 3

The effects of mitochondrial inhibitors, antimycin, dinitrophenol, nigericin and oligomycin, each used at 10μM for 3 h, on efflux of [³H]cholesterol to apoA-I (20 μg ml^-1) is shown in Figure3A. Where indicated, macrophages were preincubated for 24 h in the presence of dibutyryl cAMP (0.3 mM) to induce expression of ABCA1 and cholesterol efflux[25]. Values are the mean ± SEM of between four to six independent experiments; **p<0.01 compared with control macrophages; other significant changes are indicated on the figure. Expression of ABCA1 protein under the conditions noted is shown in Figure3B; values are shown relative to the housekeeping gene, GAPDH, and are representative of three independent experiments.