Table 4 Sequence information on the primers used for RT-PCR.
From: Role of extrathyroidal TSHR expression in adipocyte differentiation and its association with obesity
Genes | Sequences | Product size (bp) | Annealing temperature (°C) | Gene Bank |
|---|---|---|---|---|
Mouse ALBP | 5'-GATGCCTTTGTGGGAACCTGG-3' 5'-TTCATCGAATTCCACGCCCAG-3' | 213 | 55 | NM_024406.2 |
Mouse PPAR-γ | 5'-GACCACTCGCATTCCTTT-3' 5'-GGCATTGTGAGACATCCC-3' | 459 | 55 | NM_001127330.1 |
Mouse Tshr | 5'-TCATTGCCTCTGTAGACCTG-3' 5'-TGATAACTCACTGGCGAAA-3' | 119 | 53 | NM_011648.5 |
GAPDH | 5'-CAAGGTCATCCATGACAACTTTG-3' 5'-GTCCACCACCCTGTTGCTGTAG-3' | 496 | 60 | Complementary to human, mouse and rat GAPDH |