Table 1 Primers for real-time quantitative PCR analysis
From: Fuzheng Huayu recipe prevents nutritional fibrosing steatohepatitis in mice
Gene | Product length | Primer sequences |
|---|---|---|
HO-1 | 427 bp | F 5'-AACAAGCAGAACCCAGTCTATG-3' |
| Â | Â | R 5'-TGAGCAGGAAGGCGGTCTTA-3' |
CYP2E1 | 199 bp | F 5'-AACAGAGACCACCAGCACA-3' |
| Â | Â | R 5'-GGAAGGGACGAGGTTGATGA-3' |
TNF-α | 79 bp | F 5'-GAACTGGCAGAAGAGGCACT-3' |
| Â | Â | R 5'-AGAAGAGGCTGAGACATAGGC-3' |
IL-6 | 85 bp | F 5'-TACCACTCCCAACAGACCTG-3' |
| Â | Â | R 5'-TCTCATTTCCACGATTTCCCAG-3' |
α-SMA | 162 bp | F 5'-ATGATGCTCCCAGGGCTGTT -3' |
| Â | Â | R 5'-TGGTGATGATGCCGTGTTCT-3' |
TGF-β1 | 85 bp | F 5'-CCGCAACAACGCCATCTATG -3' |
| Â | Â | R 5'-TGCTTCCCGAATGTCTGACG-3' |
Col-1 | 108 bp | F 5'-TGACTGGAAGAGCGGAGAGT-3' |
| Â | Â | R 5'-GTAGGGAACACACAGGTCTGA-3' |
Col-3 | 61 bp | F 5'-CTGGTGCTAAGGGTGAAGTTG-3' |
| Â | Â | R 5'-TGTCCTGGTGAGCCATTTGAG-3' |
GAPDH | 120 bp | F 5'-TGAACGGGAAGCTCACTGG-3' |
| Â | Â | R 5'-GCTTCACCACCTTCTTGATGTC-3' |