Figure 5From: Impairment of the cell-to-matrix adhesion and cytotoxicity induced by the Mediterranean jellyfish Pelagia noctiluca venom and its fractions in cultured glioblastoma cellsU87 cells were preincubated without (control) or with 50, 50, 100, 50 and 100 μg/ml of venom, F1, F2, F3 and F4 respectively for 30 min at room temperature. Cells were then added to 96-well microtiter plates coated with 10 mg/ml with 50 mg/ml fibrinogen (Fg) or poly-L-lysine (Poly-L) and allowed to adhere for 1 h at 37°C. After washing, adherent cells were fixed, stained with crystal violet, solubilized by SDS and the absorbance was measured at 600 nm. **p < 0.01, ***p < 0.001 compared with control (untreated cells).Back to article page