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Figure 5 | Lipids in Health and Disease

Figure 5

From: Impairment of the cell-to-matrix adhesion and cytotoxicity induced by the Mediterranean jellyfish Pelagia noctiluca venom and its fractions in cultured glioblastoma cells

Figure 5

U87 cells were preincubated without (control) or with 50, 50, 100, 50 and 100 μg/ml of venom, F1, F2, F3 and F4 respectively for 30 min at room temperature. Cells were then added to 96-well microtiter plates coated with 10 mg/ml with 50 mg/ml fibrinogen (Fg) or poly-L-lysine (Poly-L) and allowed to adhere for 1 h at 37°C. After washing, adherent cells were fixed, stained with crystal violet, solubilized by SDS and the absorbance was measured at 600 nm. **p < 0.01, ***p < 0.001 compared with control (untreated cells).

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