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Table 1 Primer sequences for real-time quantitative PCR

From: The effects of berberine on hyperhomocysteinemia and hyperlipidemia in rats fed with a long-term high-fat diet

Gene

Forward primer (5’- 3’)

Reverse primer (5’- 3’)

LDLR

CCAGTGCGGCGTAGGATT

GGGACTCATCGGAGCCAT

HMGR

AGAATATAGCGCGTGGGATG

GACATACAGCCAAAGCAGCA

apoB

TAAATGGAGCACTTTTCAAG

GGAACAGCAGCAGTAGCG

apoAI

GCCACTGTGTATGTGGATGC

AACCCAGAGTGTCCCAGTTG

apoE

AGGAGCAGACCCAGCAGATA

GGAGTTGGTAGCCACAGAGG

SR

GCCACTGGTCCTTGTTTGTT

TGGGAGCGCTGACTTTTACT

beta-actin

GATTACTGCCCTGGCTCCTA

TCATCGTACTCCTGCTTGCT

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Lipids in Health and Disease

ISSN: 1476-511X