Figure 10

HDL was isolated from plasma of 50 patients with HIV infection and 50 healthy subjects, using ultracentrifugation and PEG precipitation as described in Methods. Oxidation of DHR in the presence of these 100 different HDL samples was assessed as described in Methods, using 2.5 μg (cholesterol) of added HDL. The slope of oxidation of each sample was normalized as ratio to the DOR of a control pool of HDL isolated from plasma of 50 healthy control subjects using ultracentrifugation and PEG precipitation as described in Methods. The normalized DOR (nDOR) is shown on the y axis. The data (means of quadruplicates) from three independent experiments are plotted. The median nDOR value from HIV-infected subjects was significantly (p < 0.001) higher (1.31; IQR: 1.18, 1.45) compared to the median nDOR value from healthy subjects (0.87; IQR: 0.79, 0.95) when HDL was purified using ultracentrifugation. This study had ≥ 85% study power to detect a 50% difference of nDOR (as a measure of HDL function) between patients with systemic inflammation (such as HIV infected subjects) and healthy subjects using non-parametric tests for independent samples at alpha 0.05 (two-sided).