Figure 2From: Effects of lipid-probe interactions in biochemical fluorometric methods that assess HDL redox activityDose-dependent decrease in DCF oxidation rate (as measured by changes in fluorescence per minute over 50 minutes) by incubation with increasing concentration of HDL (triangles) and LDL (squares) that were isolated by FPLC from a healthy donor, oxPAPC (circles) that was prepared as described in Materials and Methods and stable amount of DCFH (200 ug/ml). The slope of oxidation of each sample was normalized to the slope of oxidation of DCF and the % relative oxidation slope and quenching is shown on the y axis. The correlation between the relative % slope and the added amount of lipids is shown. The values represent means ± SD of quadruplicate samples from 3 independent experiments.Back to article page