Figure 6From: Effects of lipid-probe interactions in biochemical fluorometric methods that assess HDL redox activityThe effect of purified, commercially available, lipid-free apolipoprotein AI (ApoAI) from a healthy donor on DCF oxidation rate was assessed by incubation of 200 ug/ml of DCFH with increasing concentration of ApoAI (0.5 -10.0 mg/dl). The slope of oxidation of each sample was normalized to the slope of oxidation of DCF and the % relative oxidation slope and quenching is shown on the y axis as described in Figure 5A. The values represent means ± SD of quadruplicate samples. There was no concentration dependent effect of apoAI (at concentration > 2 mg/dl) on DCFH oxidation rate.Back to article page