Figure 3

The effect of PUFAs on ERK1/2 activation in Caco-2 cells. (A) Cells were treated with 100 μM EPA, DHA and AA for 20 min. Differences in pERK 1/2 activity were determined using densitometry compared to untreated cells (n = 3, * p < 0.05, ** p < 0.01). (B) Cells were treated with 100 μM EPA and DHA from 0–60 min, and 100 μM AA from 0–90 min. (C) Cells were pretreated for 1 h with 10 μM H89, 10 μM GW5074 and 100 nM Iressa before treatment with 100 μM EPA for 20 min. The lysates were subjected to Western blotting to detect phosphorylated ERK1/2 and total actin. The figure is representative for at least three experiments with similar results.