Skip to main content

Advertisement

Table 1 Primers used for quantitative RT-PCR analysis

From: Activation of peroxisome proliferator activated receptor alpha ameliorates ethanol mediated liver fibrosis in mice

Gene Product length Primer sequences
PPARα 149 bp F 5’-GATGTCACACAATGCAATTCG -3’
R 5’-GGTAGGCTTCGTGGATTCTCT -3’
TNF-α 300 bp F 5’-GGCAGGTCTACTTTGGAGTCATTGC-3’
R 5’-ACATTCGAGGCTCCAGTGAATTCGG-3’
IL-10 142bp F 5’-TGCTGCCTGCTCTTACTGAC-3’
R 5’-AGAAAGTCTTCACCTGGCTGA-3’
OPN 121 bp F 5’-GGTGATAGCTTGGCTTATGGAC-3’
R 5’-CCTTAGACTCACCGCTCTTCAT-3’
TGF-β1 272 bp F 5’-CAACGCCATCTATGAGAAAACC-3’
R 5’ -ACTGCCGTACAACTCCAGTGAC-3’
visfatin 172 bp F 5’- GGAAAGACCATGAGAAAGATGC-3’
R 5’-CTGATGATTAGTGGTGCCTCTG-3’
PI3K 198 bp F 5’- GCACGGCGATTACACTCTTAC-3’
R 5’-TTGGACACTGGGTAGAGCAAC-3’
MMP-2 203 bp F 5’- TTGTGCTGAAAGATACCCTCAA-3’
R 5’-CAGGTCAGGTGTGTAACCAATG-3’
MMP-9 242 bp F 5’-TGAATCAGCTGGCTTTTGTG-3’
R 5’-GTGGATAGCTCGGTGGTGTT-3’
adiponectin 202 bp F 5’-CCAGTATCAGGAAAAGAATGTGG-3’
R 5’-TGGTGTATGGGCTATGGGTAGT-3’
HO-1 427 bp F 5’-AACAAGCAGAACCCAGTCTATG-3’
R 5’-TGAGCAGGAAGGCGTCTTA-3’
GAPDH 233 bp F 5’-GGTGAAGGTCGGTGTGAACG-3’
   R 5’-CTCGCTCCTGGAAGATGGTG-3’
  1. Abbreviations: PPARα, peroxisome proliferator activated receptor alpha; TNF-α, tumor necrosis factor-alpha; IL-10, interleukin-10; OPN, osteopontin; TGF-β1, transforming growth factor-beta 1; PI3K, phosphatidylinositol 3-kinase; MMP-2, matrix metalloproteinase-2; MMP-9, matrix metalloproteinase-9; HO-1, heme oxygenase-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.